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| Product Name | Fast One-Tube One-Step RT-qPCR Probe Premix |
| Catalog No. | MDKPK-0036 |
| Description | A fully premixed one-tube probe RT-qPCR reagent that allows primers and probes to be incorporated in advance for simplified sample addition and rapid testing. |
| Intended Use | Diagnostic pathogen testing, animal pathogen testing and food microbiology testing. |
| Principle / Technology | Warm-start reverse transcription, hot-start Taq chemistry and an optimized fast buffer support one-step amplification. dUTP/UDG chemistry reduces carryover contamination. |
| Detection Method | Fluorescent probe-based one-step RT-qPCR. |
| Sample Type | RNA templates and compatible nasopharyngeal swab lysates. |
| Performance Range / Specifications | 200, 1,000 or 5,000 reactions; approximately 22-minute amplification; primers and probes may be pre-combined with the premix; compatible with singleplex and multiplex testing. |
| Sensitivity / LOD | Designed for stable detection of low-concentration samples. |
| Specificity | Controlled enzyme activation and optimized chemistry improve specificity during fast amplification. |
| Reaction Conditions / Protocol | Pre-combine assay primers and probes with the premix where appropriate, then add sample and run a compatible fast one-step RT-qPCR program. |
| Components / Formulation | One-tube concentrated premix containing reverse-transcription enzymes, hot-start PCR chemistry, buffer and dUTP/UDG contamination-control reagents. |
| Package Specifications | 200 reactions; 1,000 reactions; 5,000 reactions. |
| Product Form | One-tube one-step RT-qPCR probe premix. |
| Quality Control | Premix stability, rapid amplification, direct-lysate compatibility and multiplex performance are evaluated. |
| Key Features | Primer/probe preloading; approximately 22-minute detection; one-tube workflow; direct-lysate tolerance; carryover contamination control. |
| Expiration Date / Stability | Reported primer/probe premix stability reached 7 days at 37°C and 1 year at 4°C in accelerated evaluations. |
| Compatibility | Suitable for probe-based real-time PCR instruments and fast testing workflows. |
| Application Notes / Precautions | Validate the intended primer/probe combination and storage conditions before routine use as a preloaded working reagent. |
For research use only, not for clinical use.
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