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| Product Name | Fast One-Step RT-qPCR Probe Kit for DNA/RNA Co-Detection |
| Catalog No. | MDKPK-0032 |
| Description | A fast probe-based one-step RT-qPCR kit optimized for rapid RNA detection, multiplex amplification and balanced co-detection of DNA and RNA targets. |
| Intended Use | Rapid testing, virus and pathogen detection, fusion-gene analysis, DNA/RNA co-detection, gene-expression analysis, residue testing and low-copy target analysis. |
| Principle / Technology | A warm-start reverse transcriptase and antibody-modified Taq polymerase are combined with a fast-cycling buffer and dUTP/UDG carryover-control system. |
| Detection Method | Probe-based real-time RT-qPCR. |
| Sample Type | Purified RNA, DNA/RNA mixtures and compatible crude nasopharyngeal swab lysates. |
| Performance Range / Specifications | 200, 1,000 or 10,000 reactions; completes amplification in approximately 30 minutes; supports singleplex and multiplex assays and balanced DNA/RNA amplification. |
| Sensitivity / LOD | Designed for stable detection of low-concentration samples; reported low-level sample detection exceeded 95% in two multiplex systems. |
| Specificity | Hot-start enzyme regulation and an optimized fast buffer reduce nonspecific amplification. |
| Reaction Conditions / Protocol | Combine the kit components with assay-specific primers/probes and template and run a compatible fast one-step RT-qPCR program. |
| Components / Formulation | Fast one-step RT-qPCR reaction chemistry containing reverse transcriptase, antibody-regulated Taq polymerase, optimized buffer and dUTP/UDG carryover-control reagents. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 1,000 reactions; 10,000 reactions. |
| Product Form | Two-component fast RT-qPCR probe kit. |
| Quality Control | Fast cycling, low-copy detection, crude-lysate compatibility, multiplex balance and DNA/RNA co-detection are evaluated. |
| Key Features | Approximately 30-minute amplification; low-template sensitivity; crude-lysate tolerance; multiplex capability; DNA/RNA co-detection; contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for probe-based real-time PCR instruments and fast cycling workflows. |
| Application Notes / Precautions | For direct lysate testing, validate sample input and inhibitor tolerance in the intended assay. |
For research use only, not for clinical use.
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