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| Product Name | Fast Cell Direct Lysis Set (for SYBR Green RT-qPCR) |
| Catalog No. | RESK-0065 |
| Description | The Fast Cell Direct Lysis Set is designed for rapid preparation of a wide variety of animal cells—including adherent and suspension cell lines, primary cultured cells, stem cells, and iPS cells—for direct gene expression analysis without RNA extraction. The streamlined workflow completes template preparation, reverse transcription, and qPCR analysis in as little as 1.5 hours, with simple operation and a low error rate. This set serves as the cell lysis module and supplementary reagent for the dye-based Cell Direct RT-qPCR Kit, and is compatible with the vast majority of real-time fluorescence quantitative PCR instruments using universal ROX normalization. |
| Intended Use | Direct lysis of various animal cells (adherent and suspension cell lines, primary cultured cells, stem cells, iPS cells) for SYBR Green-based cell-direct RT-qPCR gene expression analysis without prior RNA extraction. |
| Principle / Technology | Cells are lysed directly with a dedicated lysis buffer, residual genomic DNA is removed by DNase I digestion, and the lysate is used directly as template for reverse transcription and SYBR Green dye-based qPCR analysis. |
| Detection Method | SYBR Green dye-based fluorescence detection on real-time quantitative PCR instruments; compatible with most qPCR platforms using universal ROX. |
| Sample Type | Cultured animal cells: adherent and suspension cell lines, primary cultured cells, various stem cells, and iPS cells. |
| Performance Range / Specifications | Complete workflow from cell template preparation to gene expression analysis in as little as 1.5 hours; antibody-based hot-start compatible qPCR workflow; suitable for most fluorescence quantitative PCR instruments. |
| Reaction Conditions / Protocol | Lyse cells with FCD Lysis Buffer, wash with FCD Washing Buffer, terminate lysis with FCD Stop Solution, and digest genomic DNA with DNase I; the resulting lysate is used directly for reverse transcription and SYBR Green qPCR. |
| Components / Formulation | FCD Lysis Buffer: 2 mL (40 T) / 5 mL (100 T) FCD Washing Buffer: 8 mL (40 T) / 20 mL (100 T) FCD Stop Solution: 100 μL (40 T) / 250 μL (100 T) DNase I: 80 μL (40 T) / 200 μL (100 T) |
| Storage Conditions | Store FCD Lysis Buffer and FCD Washing Buffer unopened at -25°C to -15°C; after thawing, keep at 4°C and protect from contamination. Store all other components at -25°C to -15°C for long-term storage. |
| Shelf Life | 6 months. |
| Package Specifications | 40 T; 100 T |
| Product Form | Kit (liquid reagents) |
| Expiration Date / Stability | Valid for 6 months when stored as recommended. |
| Compatibility | Supplementary lysis module for the dye-based Cell Direct RT-qPCR Kit; compatible with the vast majority of real-time fluorescence quantitative PCR instruments. |
| Application Notes / Precautions | This set is the cell lysis module of the dye-based Cell Direct RT-qPCR Kit and is intended as a supplementary reagent; refer to the complete kit for full product performance details. After thawing, keep FCD Lysis Buffer and FCD Washing Buffer at 4°C and take care to prevent contamination. |
For research use only, not for clinical use.
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