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| Product Name | EPS-Based Alpha-Amylase Reagent for 405 nm Serum and Plasma AMY Testing |
| Catalog No. | BBITR-0001 |
| Description | Alta DiagnoTech alpha-amylase reagent is a two-reagent EPS substrate system for quantitative in vitro measurement of alpha-amylase activity in human serum or plasma. |
| Intended Use | In vitro quantitative determination of alpha-amylase activity in human serum or plasma; intended to support auxiliary assessment and treatment monitoring of acute pancreatitis. |
| Principle / Technology | Alpha-amylase cleaves ethylidene-p-nitrophenyl-alpha-D-maltoheptaoside. Alpha-glucosidase then hydrolyzes the degradation products to p-nitrophenol, whose color intensity at 405 nm is proportional to alpha-amylase activity. |
| Detection Method | Kinetic p-nitrophenol colorimetric assay at 405 nm |
| Sample Type | Human serum or plasma |
| Components / Formulation | Reagent 1: HEPES 50 mmol/L, sodium chloride 70 mmol/L, magnesium chloride 10 mmol/L and alpha-glucosidase >=4 kU/L. Reagent 2: EPS 3.5 mmol/L. |
| Storage Conditions | Store unopened or opened reagent protected from light at 2-8 degrees C; do not freeze. |
| Shelf Life | 12 months unopened when sealed and protected from light at 2-8 degrees C |
| Application Notes / Precautions | Do not mix reagent lots; recalibrate when changing lots. |
| Alias | Alpha-Amylase (AMY) Reagent |
| Product Type | Alpha-amylase biochemical test reagent |
| Function / Efficacy | Support quantitative colorimetric measurement of alpha-amylase activity. |
| Abbreviation | AMY reagent |
| Detection Wavelength | 405 nm |
For research use only, not for clinical use.
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