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| Product Name | Epoxy-Activated Magnetic Beads for Covalent Ligand Coupling (2 µm) |
| Catalog No. | MAGBEA-0021 |
| Description | Epoxy-functionalized superparamagnetic microspheres designed for covalent immobilization of ligands via their primary amine, hydroxyl, or sulfhydryl groups to reactive epoxy moieties on the bead surface. The covalent linkage withstands harsh elution conditions and prevents ligand leaching during repeated use. These beads serve as a versatile platform for preparing custom affinity matrices by coupling user-selected antibodies, proteins, peptides, or small molecules. |
| Intended Use | Covalent immobilization of affinity ligands for custom magnetic separation applications in immunoprecipitation and protein purification. |
| Principle / Technology | Epoxy-amine covalent coupling chemistry with stable ether bond formation |
| Detection Method | Protein quantification of immobilized ligand by BCA assay; binding capacity verification with fluorescent ligand partner |
| Sample Type | User-supplied antibodies, recombinant proteins, peptides, or small molecule ligands containing primary amine, sulfhydryl, or hydroxyl groups |
| Performance Range / Specifications | Ligand coupling capacity: ≥200 µg IgG per mg beads; particle diameter: 2 µm; surface epoxy group density: ≥50 µmol/g beads |
| Sensitivity / LOD | Coupled ligand activity retention: ≥80% of free ligand binding activity after immobilization |
| Specificity | Reactive to primary amines (optimal at pH 8–10), sulfhydryls (pH 7–9), and hydroxyls (pH 11–13) |
| Reaction Conditions / Protocol | Wash beads with coupling buffer; incubate with ligand in coupling buffer 16 hr at 4 °C or 4 hr at room temperature with gentle rotation; block remaining active groups with 1 M ethanolamine; wash; store in PBS with 0.02% sodium azide at 2–8 °C |
| Components / Formulation | Epoxy-activated magnetic bead suspension (10 mg/mL in isopropanol), coupling buffer concentrate, blocking solution (1 M ethanolamine), wash/storage buffer |
| Storage Conditions | Beads: 2–8 °C |
| Shelf Life | 18 months at 2–8 °C |
| Package Specifications | 2 mL bead suspension (20 mg beads) |
| Product Form | Magnetic bead suspension in isopropanol |
| Quality Control | Ligand coupling capacity verified with rabbit IgG per lot; free epoxy group content quantified by thiosulfate titration |
| Key Features | Covalent linkage stability enables repeated bead regeneration and reuse cycles without ligand activity loss |
| Purity | Endotoxin ≤0.1 EU/mg; heavy metal content ≤10 ppm |
| Concentration | As specified (mg/mL suspension) |
| Activity / Unit Definition | Binding capacity in µg target per mg beads as specified |
| Molecular Weight | Not applicable — inorganic/organic composite particles |
| Source / Origin | Synthetic polymer-magnetite composite; recombinant protein ligands where applicable |
| pH Range / Optimal pH | pH 6.0–9.0 working range; optimal pH varies by surface chemistry |
| Shipping Conditions | Ambient temperature; protect from freezing for aqueous suspensions |
| Expiration Date / Stability | 18–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; ISO 9001 manufacturing; custom GMP options available |
| Compatibility | Compatible with manual magnetic racks and automated KingFisher and similar platforms |
| Recommended Buffer System | PBS pH 7.4 with 0.02% sodium azide or 20% ethanol for long-term storage |
| Application Notes / Precautions | Vortex thoroughly before each use; avoid drying beads; use appropriate magnetic separation times |
| Batch-to-Batch Consistency | Particle size CV ≤10%; binding capacity within ±15% of reference lot |
For research use only, not for clinical use.
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