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Enhanced Red Fluorescence TUNEL Apoptosis Detection Kit

Cat.No: CAAK-0005 Datasheet

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Product Name Enhanced Red Fluorescence TUNEL Apoptosis Detection Kit
Catalog No. CAAK-0005
Description A red-fluorescence TUNEL assay incorporating a signal-enhancing small molecule for brighter labeling and improved resistance to photobleaching.
Intended Use Detection of apoptosis in paraffin sections, frozen sections, adherent cultured cells and suspension cultured cells.
Principle / Technology Recombinant TdT labels exposed DNA 3′-OH ends with a red fluorescent nucleotide mixture, while a fluorescence-enhancing factor increases brightness and stability.
Detection Method Fluorescence microscopy or compatible fluorescence-analysis platforms.
Sample Type Paraffin sections, frozen sections, adherent cells and suspension cells.
Performance Range / Specifications Available for 20, 50 or 100 reactions.
Reaction Conditions / Protocol Fix and permeabilize samples, perform TdT-mediated red-fluorescence labeling, wash and analyze while protecting the reaction from light.
Components / Formulation High-activity recombinant TdT enzyme and enhanced red fluorescent labeling mixture.
Storage Conditions Store at -30 to -15°C protected from light. Protect the red labeling mixture from light.
Package Specifications 20 reactions; 50 reactions; 100 reactions.
Product Form Enhanced red-fluorescence TUNEL kit.
Key Features Bright red fluorescence; improved photostability; high-efficiency TdT labeling; compatible with green fluorescent co-staining.
Shipping Conditions Ship at 0°C or below.
Compatibility Suitable for co-labeling with GFP and other green-fluorescence markers.
Application Notes / Precautions Protect fluorescent components and stained samples from light.

For research use only, not for clinical use.

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