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Enhanced Green Fluorescence TUNEL Apoptosis Detection Kit

Cat.No: CAAK-0006 Datasheet

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Product Name Enhanced Green Fluorescence TUNEL Apoptosis Detection Kit
Catalog No. CAAK-0006
Description An enhanced green-fluorescence TUNEL assay using a small-molecule signal stabilizer to increase fluorescence brightness and resistance to photobleaching.
Intended Use Detection of apoptotic DNA fragmentation in paraffin sections, frozen sections, adherent cells and suspension cells.
Principle / Technology TdT-mediated nucleotide incorporation labels exposed DNA 3′-OH ends, and a green-fluorescence enhancing factor stabilizes and amplifies the signal.
Detection Method Fluorescence microscopy or compatible fluorescence-analysis platforms.
Sample Type Paraffin sections, frozen sections, adherent cells and suspension cells.
Performance Range / Specifications Available for 20, 50 or 100 reactions.
Reaction Conditions / Protocol Fix and permeabilize samples, perform TdT-mediated green-fluorescence labeling, wash and analyze while protected from light.
Components / Formulation High-activity recombinant TdT enzyme and enhanced green fluorescent labeling mixture.
Storage Conditions Store at -30 to -15°C. Protect the green labeling mixture from light.
Package Specifications 20 reactions; 50 reactions; 100 reactions.
Product Form Enhanced green-fluorescence TUNEL kit.
Key Features High fluorescence brightness; enhanced photostability; high-efficiency TdT labeling; compatible with cultured cells and tissue sections.
Shipping Conditions Ship at 0°C or below.
Compatibility Fluorescence microscopy and other green-fluorescence detection platforms.
Application Notes / Precautions Protect fluorescent components and labeled samples from light.

For research use only, not for clinical use.

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