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| Product Name | EdU-555 Cell Proliferation Assay Kit, for FACS and FM |
| Catalog No. | REKI-0041 |
| Description | EdU nucleoside incorporation kit using an orange red azide fluorescent dye at the 555 nm channel, offered for flow cytometry and fluorescence microscopy in a 100-test format. EdU enters newly synthesized DNA during S phase and its alkyne couples to the azide dye in a copper-catalyzed click reaction, so dividing cells report directly through DNA synthesis rather than indirect metabolic proxies. The red channel placement frees the green channel for co-stains. Store at 2-8 °C; shelf life is 12 months. |
| Intended Use | DNA synthesis based proliferation detection in the orange red channel |
| Principle / Technology | EdU labels S-phase DNA, and a copper-catalyzed click reaction links the alkyne to an orange red azide probe |
| Specificity | 100 T; orange red fluorescence channel; 12 month shelf life |
| Reaction Conditions / Protocol | Pulse-label cells with EdU, fix, run the click reaction with the azide dye and acquire on the 555 nm channel |
| Storage Conditions | 2-8 °C |
| Package Specifications | 100 T |
| Key Features | Red-channel readout preserves green channels for multiplexing; antibody-free DNA synthesis detection; 12 month shelf life |
For research use only, not for clinical use.
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