- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Dialysis Membrane, Regenerated Cellulose, MWCO 3.5, 10, and 50 kDa, Assorted Kit |
| Catalog No. | IGM-HMM-0073 |
| Description | Assorted dialysis membrane kit containing pre-cut regenerated cellulose tubing with three molecular weight cut-off (MWCO) values: 3.5 kDa, 10 kDa, and 50 kDa. Dialysis is a membrane-based separation technique used for buffer exchange, desalting, removal of low molecular weight contaminants, and concentration of macromolecular solutions. The regenerated cellulose membrane is a transparent, flexible, and hydrophilic film with well-defined pore sizes that allow selective passage of molecules based on molecular weight. Molecules smaller than the MWCO pass freely through the membrane pores, while molecules larger than the MWCO are retained. This assay kit provides the three most commonly used MWCO values for protein, peptide, nucleic acid, and nanoparticle purification. Membranes are supplied as dry, flat sheets (10 sheets each of 10 x 10 cm) with glycerol as a humectant, requiring a brief pre-treatment (soaking in water) before use. |
| Intended Use | Buffer exchange and desalting of: protein solutions (antibodies, enzymes, recombinant proteins) to remove imidazole, salts, urea, guanidine, or other small molecules; peptide desalting and purification; removal of unincorporated labels (biotin, fluorophores, crosslinkers) from labeled proteins; nanoparticle and quantum dot purification; nucleic acid desalting and buffer exchange; concentration of dilute macromolecule solutions using dry absorbent polymers on the outside of the dialysis bag; and controlled drug release studies. |
| Principle / Technology | Dialysis is driven by the concentration gradient of permeable solutes across a semipermeable membrane. The regenerated cellulose membrane contains a network of tortuous pores created by the removal of cellulose acetate groups during regeneration. The pore size distribution determines the MWCO — defined as the molecular weight at which 90% of solute is retained. In practice: MWCO 3.5 kDa retains most peptides >3.5 kDa and all proteins; MWCO 10 kDa retains proteins >10 kDa (insulin, cytochrome c-sized); MWCO 50 kDa retains proteins >50 kDa (BSA, IgG-sized). Small molecules (salts, buffer ions, imidazole, urea, small ligands) diffuse freely through all three MWCO membranes, establishing equilibrium with the external buffer within hours to overnight. |
| Detection Method | 1) Cut a piece of dialysis membrane to desired length (include extra length for closure); 2) Soak membrane in ultrapure water for 30 min to remove glycerol humectant and hydrate membrane; 3) Rinse membrane thoroughly inside and out with water; 4) Seal one end with a dialysis clip or knot; 5) Fill membrane with sample using pipette or syringe, leaving a small air headspace; 6) Seal the other end with clip or knot; 7) Immerse membrane in a large excess of dialysis buffer (typically 200-1,000x sample volume) with gentle stirring at desired temperature; 8) Change dialysis buffer 2-3 times at 2-4 hour intervals (or overnight); 9) After dialysis, cut one end and recover sample with pipette. |
| Sample Type | Protein solutions (0.1-100 mg/mL), peptide solutions, nucleic acid solutions, nanoparticle suspensions; sample volume: 0.1-30 mL depending on membrane width; sample must be free of particulates (centrifuge or filter before dialysis). |
| Performance Range / Specifications | MWCO: 3.5 kDa, 10 kDa, 50 kDa (each provided separately); membrane material: regenerated cellulose; thickness: 20-40 um (dry), 40-80 um (hydrated); glycerol content: 10-25% (as humectant); protein retention: >90% for proteins >MWCO; salt clearance: >99.9% after 3 buffer changes (2-4 h each); membrane tensile strength: >10 N (wet). |
| Sensitivity / LOD | Buffers exchange: removal of >99.9% NaCl after 3 changes of 500x volume; removal of >99% imidazole, urea, guanidine-HCl after 3 buffer changes; protein recovery: >95% for proteins well below MWCO. |
| Specificity | Regenerated cellulose membrane is hydrophilic and has very low non-specific protein binding (<1 ug/cm^2 for most proteins); pore size (MWCO) determines molecular size selectivity; membrane is permeable to water, salts, buffer ions, and small organic molecules (urea, guanidine, sugars, amino acids, ATP, cofactors); membrane is impermeable to macromolecules larger than the MWCO. |
| Reaction Conditions / Protocol | Dialysis at 4 C or RT (4 C recommended for labile proteins — reduces proteolysis and microbial growth); typical dialysis time: 4-24 hours (equilibrium reached within 2-4 hours for small molecules with stirring); stir dialysis buffer for efficient mass transfer; buffer changes needed to overcome equilibrium limitations. |
| Components / Formulation | Regenerated Cellulose Dialysis Membrane, MWCO 3.5 kDa: 10 sheets, 10 x 10 cm; MWCO 10 kDa: 10 sheets, 10 x 10 cm; MWCO 50 kDa: 10 sheets, 10 x 10 cm; Dialysis Clips (polypropylene, 20 clips, assorted colors for each MWCO); Instruction Manual. |
| Storage Conditions | Store dry membranes at RT (15-25 C) in sealed plastic bag with desiccant; protect from dust, moisture, and direct sunlight. |
| Shelf Life | 36 months from date of manufacture at RT in original sealed packaging. |
| Package Specifications | Assorted Kit: 10 sheets each of 3.5, 10, and 50 kDa MWCO (30 sheets total), each 10 x 10 cm, plus 20 dialysis clips. |
| Product Form | Transparent, flexible dry regenerated cellulose sheets (10 x 10 cm), with glycerol humectant, individually interleaved with protective paper. |
| Quality Control | Each lot tested: MWCO accuracy (protein retention: >90% for proteins >MWCO, <10% retention for proteins <0.5x MWCO); membrane thickness 20-40 um dry; glycerol content 10-25%; tensile strength >10 N wet; absence of pinholes (leak test with dye solution); protein adsorption <1 ug BSA/cm^2; DNase/RNase: not detected; heavy metals: <10 ppm total. |
| Key Features | Assorted kit with three MWCO values (3.5, 10, 50 kDa); 10 sheets each; regenerated cellulose — low protein binding; dialysis clips included; hydrophilic — no pre-wetting with organic solvents; dry storage with glycerol humectant; DNase/RNase free. |
| Purity | Regenerated cellulose: >99% pure cellulose; glycerol: USP grade; no surfactants, plasticizers, or preservatives; heavy metals <10 ppm total. |
| Concentration | Not applicable; membrane material with defined MWCO. |
| Activity / Unit Definition | MWCO accuracy confirmed by protein retention assay; tensile strength >10 N wet. |
| Molecular Weight | Cellulose: (C6H10O5)n; pore size corresponding to 3.5 kDa approximately 1.5-2.5 nm, 10 kDa approximately 2.5-4 nm, 50 kDa approximately 4-7 nm (hydrated). |
| Source / Origin | Regenerated cellulose from wood pulp (cotton linters or high-alpha wood cellulose); glycerol from plant-derived source; no animal-derived components. |
| pH Range / Optimal pH | Regenerated cellulose stable at pH 2.0-12.0 for short-term use (hours); pH 4.0-9.0 recommended for extended dialysis (overnight); avoid pH <2.0 (acid hydrolysis of cellulose) and pH >12.0 (alkaline degradation). |
| Shipping Conditions | Ambient temperature; protect from moisture (sealed bag with desiccant). |
| Expiration Date / Stability | 36 months at RT in sealed bag with desiccant; after opening, reseal bag with desiccant and use within 12 months; glycerol may slowly evaporate if packaging is compromised — rehydrate dried membranes in 0.1% glycerol before use. |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic use. Not for in vivo implantation. Not for clinical dialysis of patients. |
| Compatibility | Compatible with aqueous buffers: PBS, TBS, Tris, HEPES, phosphate, carbonate, borate, acetate, and citrate buffers at pH 2-12. Compatible with denaturants: urea (up to 8 M), guanidine-HCl (up to 6 M), SDS (up to 1%). Compatible with reducing agents: DTT (up to 10 mM), beta-mercaptoethanol (up to 100 mM). Compatible with organic solvents: methanol, ethanol, isopropanol up to 20%; acetonitrile up to 10%. Not compatible with: concentrated strong acids (concentrated HCl, HNO3, H2SO4); concentrated strong bases (NaOH >1 M); oxidizing agents (hydrogen peroxide, sodium hypochlorite); cellulose-degrading enzymes (cellulase). Membrane is sensitive to microbial growth — use sterile technique and include 0.02% sodium azide in dialysis buffer for extended dialyses (>24 h) at RT. |
| Recommended Buffer System | Dialysis buffer: any aqueous buffer system within pH 2-12; typical: PBS pH 7.4, TBS pH 7.6, 50 mM Tris-HCl pH 8.0, or water. |
| Application Notes / Precautions | Before use, soak dry membranes in ultrapure water for 30 min with 2-3 water changes to remove the glycerol humectant (glycerol absorbs at 205 nm, interfering with protein UV detection). Handle hydrated membranes gently — they are more fragile than synthetic membranes. Always wear clean, powder-free gloves; never handle membranes with bare hands (fingerprint oils and proteins contaminate the membrane). For heat-sensitive samples, soak and dialyze at 4 C. Use magnetic stirring for efficient dialysis; ensure membrane does not contact the stir bar. Leave an air bubble (10-20% of bag volume) in the dialysis bag to allow expansion due to osmotic pressure. The choice of MWCO impacts dialysis time: use MWCO at least 3x the molecular weight of the molecule to be retained for efficient small molecule removal. For concentrating samples: place the filled dialysis bag on a bed of dry PEG 20,000 or sucrose powder, or use centrifugal concentrators — the water is drawn out osmotically while macromolecules are retained. Used membranes should not be dried for reuse (pores may collapse irreversibly upon drying after initial hydration). |
| Batch-to-Batch Consistency | MWCO accuracy confirmed for every lot (protein retention 90% at rated MWCO); membrane thickness 20-40 um; glycerol content 10-25%; pinhole-free; protein binding <1 ug/cm^2. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
