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| Product Name | Concentrated One-Tube RT-qPCR Probe Master Mix |
| Catalog No. | MDKPK-0039 |
| Description | A concentrated one-tube probe RT-qPCR master mix designed for sensitive singleplex and multiplex detection of RNA templates. |
| Intended Use | Virus detection, pathogen testing, fusion-gene analysis, DNA/RNA co-detection and gene-expression analysis. |
| Principle / Technology | A concentrated master mix combines reverse transcriptase, hot-start Taq polymerase, optimized buffer and thermolabile UDG carryover-control chemistry. |
| Detection Method | TaqMan-type and other fluorescent probe-based real-time RT-qPCR. |
| Sample Type | RNA templates, including viral and cellular RNA. |
| Performance Range / Specifications | 100, 1,000 or 10,000 reactions at 20 µL per reaction; supports singleplex and multiplex assays and maintains balanced amplification across high and low template levels. |
| Sensitivity / LOD | Reported detection reached 0.1 pg total RNA or fewer than 10 copies of RNA template. |
| Specificity | Hot-start polymerase regulation and optimized probe chemistry support specific amplification. |
| Reaction Conditions / Protocol | Combine the 5× master mix with assay-specific primers/probes and RNA template and run a compatible one-step RT-qPCR program. |
| Components / Formulation | 5× one-step probe RT-qPCR master mix containing reverse transcriptase, thermolabile UDG, hot-start Taq polymerase and optimized reaction buffer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 1,000 reactions; 10,000 reactions (20 µL per reaction). |
| Product Form | Concentrated one-tube RT-qPCR probe master mix. |
| Quality Control | Low-copy sensitivity, multiplex performance, high/low-template balance, freeze–thaw stability and transport stability are evaluated. |
| Key Features | 5× concentrated format; one-tube workflow; high sensitivity; multiplex compatibility; dUTP/UDG carryover control; strong stability. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported performance remained stable after 4 weeks at 4°C, 7 days at 37°C, up to 50 freeze–thaw cycles and a 7-day transport stress test. |
| Compatibility | Suitable for fluorescent probe-based real-time PCR instruments. |
| Application Notes / Precautions | Avoid repeated contamination exposure during setup and include appropriate controls for low-copy assays. |
For research use only, not for clinical use.
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