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| Product Name | Colorimetric LAMP/RT-LAMP Master Mix |
| Catalog No. | MDKPK-0046 |
| Description | A one-tube LAMP and RT-LAMP premix that combines visual color interpretation with optional real-time fluorescence monitoring. |
| Intended Use | Rapid isothermal detection of DNA or RNA targets. |
| Principle / Technology | Warm-start Bst polymerase and reverse transcriptase operate in an optimized buffer containing fluorescent and colorimetric dyes. Positive reactions change from red to yellow, while dUTP/UDG chemistry limits carryover contamination. |
| Detection Method | Visual color change and real-time fluorescence. |
| Sample Type | DNA or RNA templates used in LAMP or RT-LAMP assays. |
| Performance Range / Specifications | 100 or 500 reactions; typical detection is completed within 30 minutes. |
| Sensitivity / LOD | Designed for sensitive low-template LAMP and RT-LAMP detection. |
| Specificity | Warm-start enzyme control and optimized chemistry reduce nonspecific amplification; no-template reactions remained negative in the reported evaluations. |
| Reaction Conditions / Protocol | Add assay-specific primers and template to the one-tube premix, incubate under validated LAMP or RT-LAMP conditions, and interpret red as negative and yellow as positive. |
| Components / Formulation | One-tube master mix containing Bst DNA polymerase, reverse transcriptase, optimized buffer, fluorescent dye, visual indicator dye, dUTP and UDG. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Colorimetric and fluorescent LAMP/RT-LAMP master mix. |
| Quality Control | Amplification speed, sensitivity, specificity, visual color contrast and carryover-control performance are evaluated. |
| Key Features | One-tube workflow; visual readout; real-time fluorescence option; approximately 30-minute detection; room-temperature setup; contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for LAMP and RT-LAMP workflows with visual or fluorescent readout. |
| Application Notes / Precautions | Keep reaction tubes closed after amplification to minimize aerosol contamination. |
For research use only, not for clinical use.
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