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| Product Name | Citric Acid-Sodium Citrate Buffer, 0.1 M, pH 4.5 |
| Catalog No. | ATR-B-YJL-0168 |
| Description | A low-pH buffer system composed of citric acid and trisodium citrate, providing buffering capacity in the acidic range for antigen retrieval and enzymatic digestion protocols. |
| Intended Use | Heat-induced epitope retrieval in immunohistochemistry, acid phosphatase assays, and pH-specific enzymatic digestion of proteins. |
| Principle / Technology | Polyprotic acid buffer system with three pKa values (3.13, 4.76, 6.40) allowing flexible buffering across the acidic pH spectrum. |
| Detection Method | pH electrode |
| Sample Type | Formalin-fixed paraffin-embedded tissue sections, protein preparations, cell lysates |
| Performance Range / Specifications | pH 4.3–4.7, adjustable formulation for pH 3.0–6.2 |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use undiluted for antigen retrieval at 95–100°C for 10–30 minutes; dilute as needed for enzymatic assays. |
| Components / Formulation | Citric acid monohydrate, trisodium citrate dihydrate in ultrapure water |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid |
| Quality Control | pH verification, heavy metal testing, DNase/RNase free |
| Key Features | Standard for IHC antigen retrieval; adjustable pH range; compatible with microwave and water bath heating |
For research use only, not for clinical use.
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