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| Product Name | Cell Mitochondria Isolation Kit |
| Catalog No. | REKI-0045 |
| Description | Mitochondrial fractionation from cultured cells has two prizes: intact organelles and the cytosolic protein left behind, and this kit captures both in a single workflow. Most recovered mitochondria keep their inner and outer membranes intact and remain physiologically competent, supporting readouts as demanding as membrane potential measurement. The cytosolic fraction suits analysis of cytochrome c and other proteins released from mitochondria during apoptosis, and both fractions carry through to SDS-PAGE, Western blotting and two-dimensional electrophoresis. |
| Intended Use | Simultaneous isolation of functional mitochondria and mitochondria-free cytosolic protein from cultured cells. |
| Principle / Technology | Differential disruption and fractionation separate intact mitochondria from the cytosolic compartment. |
| Specificity | 50 tests per kit; yields intact mitochondria and cytosolic protein fraction. |
| Reaction Conditions / Protocol | Follow the kit manual for cell disruption, fractionation and downstream protein analysis. |
| Storage Conditions | -20°C; 12 months. |
| Package Specifications | 50T |
| Key Features | Dual readout design; membrane-intact mitochondria; fractions ready for Western blot and membrane potential assays. |
For research use only, not for clinical use.
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