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Tissue Mitochondria Isolation Kit

Cat.No: REKI-0046 Datasheet

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Product Details Related Products
Product Name Tissue Mitochondria Isolation Kit
Catalog No. REKI-0046
Description Extending the fractionation workflow to tissue samples, this kit isolates mitochondria quickly enough to preserve their physiology while simultaneously delivering the mitochondria-depleted cytosolic fraction. Intact inner and outer membranes survive in most recovered organelles, so membrane potential assays remain on the menu, and the cytosolic side supports tracking of cytochrome c release in apoptotic studies. Protein from either fraction feeds SDS-PAGE, Western blotting and 2D electrophoresis without further cleanup.
Intended Use Isolation of functional mitochondria and cytosolic protein from animal tissue samples.
Principle / Technology Rapid tissue disruption followed by differential fractionation preserves mitochondrial integrity.
Specificity 50 tests per kit; intact mitochondria plus cytosolic protein fraction.
Reaction Conditions / Protocol Process the tissue according to the kit manual and analyse fractions by the chosen downstream method.
Storage Conditions -20°C; 12 months.
Package Specifications 50T
Key Features Tissue-scale format; physiology-preserving speed; dual fractions for apoptosis and proteomics work.

For research use only, not for clinical use.

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