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| Product Name | Cell Lysis Buffer, Non-Denaturing, 1x |
| Catalog No. | ATR-B-YJL-0179 |
| Description | A mild non-denaturing lysis buffer using non-ionic detergent for extraction of soluble cytoplasmic proteins while preserving native protein conformation and enzymatic activity. |
| Intended Use | Gentle extraction of cytoplasmic proteins for enzymatic assays, co-immunoprecipitation, and studies requiring preservation of protein-protein interactions. |
| Principle / Technology | Non-ionic detergent Triton X-100 selectively permeabilizes plasma membrane while leaving nuclear and organelle membranes intact; Tris-NaCl-EDTA provides physiological ionic conditions. |
| Detection Method | Bradford protein assay, specific enzyme activity measurement, co-IP validation |
| Sample Type | Adherent and suspension cultured mammalian cells |
| Performance Range / Specifications | 95% cytoplasmic protein recovery with minimal nuclear contamination |
| Sensitivity / LOD | N/A |
| Specificity | Cytoplasmic protein enrichment; nuclear histone H3 contamination <5% |
| Reaction Conditions / Protocol | Wash cells with cold PBS; add buffer with protease inhibitors; scrape adherent cells; incubate 10 min on ice; centrifuge at 12,000 × g 10 min at 4°C. |
| Components / Formulation | Tris-HCl pH 7.5 20 mM, NaCl 150 mM, EDTA 1 mM, EGTA 1 mM, Triton X-100 1%, sodium pyrophosphate 2.5 mM, beta-glycerophosphate 1 mM, sodium orthovanadate 1 mM |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Lysis efficiency, kinase and phosphatase activity preservation verification |
| Key Features | Preserves protein interactions; compatible with kinase assays; non-denaturing conditions; validated for co-IP and enzyme activity assays |
For research use only, not for clinical use.
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