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| Product Name | Cell-Free Protein Expression Kit, Standard |
| Catalog No. | REKI-0007 |
| Description | An in vitro expression system that synthesises protein from plasmid, linear template or PCR product using an optimized E. coli extract. Solution A carries the reaction buffer with an amino-acid mixture at optimized concentrations and an ATP regeneration system that keeps feeding protein synthesis, while solution B is the engineered extract stabilising the transcription and translation machinery. Coupled one-step reactions finish in as little as one hour with yields above 3 mg/mL, a positive-control plasmid is included, and reactions scale across 96-well plates for high-throughput screening. |
| Intended Use | Rapid expression of antibodies, enzymes, membrane proteins and disulfide-rich proteins; high-throughput expression screening in 96-well plates. |
| Components / Formulation | Solution A (reaction buffer system with optimized amino acids and ATP regeneration system), solution B (optimized E. coli extract), positive-control plasmid |
| Storage Conditions | -85 to -65 °C |
| Shelf Life | 1 year |
| Package Specifications | 1 mL (20 reactions of 50 µL), 5 mL (100 reactions) |
| Product Form | Two liquid solutions (A and B) |
| Shipping Conditions | Dry ice |
| Compatibility | T7 promoter plasmids lacking the lac operator, linear templates and PCR products |
| Readouts / Downstream Assays | SDS-PAGE analysis, downstream functional assays of expressed protein |
For research use only, not for clinical use.
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