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| Product Name | Cell-Free Protein Expression Kit, Pro |
| Catalog No. | REKI-0008 |
| Description | An enhanced in vitro expression system built on the coupled transcription-translation platform with an optimized E. coli extract. Solution A provides the reaction buffer, optimized amino acids and the ATP regeneration system, and solution B is the engineered extract tuned to raise soluble protein yield, which suits membrane proteins and full-length antibodies that standard systems struggle to express. Reactions finish in as little as one hour with yields above 3 mg/mL, co-expression of several targets in one tube is supported, and plates scale to 96-well screening. |
| Intended Use | Expression of membrane proteins, full-length IgG and Fab antibodies, and disulfide-rich proteins; high-throughput screening. |
| Components / Formulation | Solution A (reaction buffer, amino acids and ATP regeneration system), solution B (optimized E. coli extract), positive-control plasmid |
| Storage Conditions | -85 to -65 °C; one component held at -25 to -15 °C; avoid repeated freeze-thaw cycles |
| Shelf Life | 1 year |
| Package Specifications | 1 mL (20 reactions of 50 µL), 5 mL (100 reactions) |
| Product Form | Two liquid solutions (A and B) |
| Shipping Conditions | Dry ice |
| Compatibility | T7 promoter plasmids lacking the lac operator, linear templates and PCR products |
| Readouts / Downstream Assays | SDS-PAGE analysis, soluble yield measurement, downstream functional assays |
For research use only, not for clinical use.
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