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| Product Name | Cell-Free Protein Expression Kit, High-Throughput |
| Catalog No. | REKI-0009 |
| Description | A high-throughput version of the coupled transcription-translation system for parallel expression screening. Solution A carries the optimized buffer, amino acids and ATP regeneration system and solution B the engineered E. coli extract, with a positive-control plasmid included for monitoring performance. Reactions complete in as little as one hour with yields above 3 mg/mL from plasmid, linear template or PCR product, and expression conditions proven in 96-well plates can be scaled up directly. |
| Intended Use | High-throughput screening of protein, enzyme and antibody expression; co-expression of multiple targets in one reaction. |
| Components / Formulation | Solution A (optimized reaction buffer, amino acids and ATP regeneration system), solution B (E. coli extract), positive-control plasmid |
| Storage Conditions | -85 to -65 °C; one component held at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 5 mL (100 reactions of 50 µL) |
| Product Form | Two liquid solutions (A and B) |
| Shipping Conditions | Dry ice |
| Compatibility | T7 promoter plasmids lacking the lac operator, linear templates and PCR products |
| Readouts / Downstream Assays | SDS-PAGE analysis, plate-reader assays, downstream functional screening |
For research use only, not for clinical use.
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