- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Casein-Based Blocking Buffer, 1x Solution |
| Catalog No. | ATR-B-YJL-0171 |
| Description | A casein-based protein blocking reagent offering superior blocking performance for nitrocellulose and PVDF membranes in chemiluminescent Western blot detection systems. |
| Intended Use | Membrane blocking for Western blotting, particularly suited for chemiluminescent detection where BSA may cause high background due to endogenous biotin or IgG contamination. |
| Principle / Technology | Casein phosphoproteins provide diverse blocking epitopes that saturate membrane binding sites more effectively than single-protein blockers for certain antibody combinations. |
| Detection Method | Western blot chemiluminescence imaging, densitometric background analysis |
| Sample Type | Nitrocellulose membranes, PVDF membranes, protein blots |
| Performance Range / Specifications | Signal-to-noise ratio improvement of 3-10 fold compared to milk-based blockers for phospho-specific antibodies |
| Sensitivity / LOD | N/A |
| Specificity | Casein-derived phosphoproteins; phosphate-free option available for phosphoprotein detection |
| Reaction Conditions / Protocol | Incubate membrane for 1 hour at room temperature or 30 minutes at 37°C with gentle agitation. |
| Components / Formulation | Purified casein hydrolysate in Tris-buffered saline, pH 7.4, preservative included |
| Storage Conditions | 2–8°C, do not freeze |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, ready to use |
| Quality Control | Membrane blocking efficiency testing, biotin contamination <0.1 ppb, phosphatase activity absent |
| Key Features | Superior for phospho-specific antibodies; low phosphatase activity; ready to use; compatible with HRP and AP detection |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
