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| Product Name | Carbonate-Bicarbonate Coating Buffer, 0.05 M, pH 9.6 |
| Catalog No. | ATR-B-YJL-0169 |
| Description | An alkaline carbonate-bicarbonate coating buffer optimized for passive adsorption of proteins onto polystyrene ELISA plate surfaces through hydrophobic and ionic interactions. |
| Intended Use | Coating buffer for immobilizing capture antibodies or antigens onto microtiter plate surfaces in indirect, sandwich, and competitive ELISA formats. |
| Principle / Technology | Alkaline pH promotes protein ionization and enhances hydrophobic binding to polystyrene surfaces; carbonate species maintain pH stability during coating incubation. |
| Detection Method | pH electrode, ELISA performance verification |
| Sample Type | Monoclonal and polyclonal antibodies, recombinant proteins, peptide antigens |
| Performance Range / Specifications | pH 9.4–9.8, carbonate concentration 0.05 M |
| Sensitivity / LOD | N/A |
| Specificity | Verification against standard coating protocol with known antibody panel |
| Reaction Conditions / Protocol | Add 100 µL per well; incubate overnight at 4°C or 2 hours at 37°C; wash before blocking. |
| Components / Formulation | Sodium carbonate, sodium bicarbonate in ultrapure water |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Quality Control | pH verification, ELISA plate coating efficiency >95% for standard IgG panel |
| Key Features | Optimized for high-binding ELISA plates; ready to use; validated with IgG and IgM antibodies |
For research use only, not for clinical use.
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