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Canine RETN (Resistin) ELISA Kit

Cat.No: HTK-YJL-0334 Datasheet

Specification Quantities

48T:
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96T:
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Product Details Background Product Features Product Advantages Related Products
Product Name Canine RETN (Resistin) ELISA Kit
Catalog No. HTK-YJL-0334
Synonyms Resistin ELISA Kit, Adipose Tissue-specific Secretory Factor ELISA Kit, ADSF ELISA Kit, C/EBP-epsilon-regulated Myeloid-specific Secreted Cysteine-rich Protein ELISA Kit, Cysteine-rich Secreted Protein A12-alpha-like 2 ELISA Kit, Cysteine-rich Secreted Protein FIZZ3 ELISA Kit, RETN ELISA Kit, FIZZ3 ELISA Kit, HXCP1 ELISA Kit, RSTN ELISA Kit
Species Canine
UniProt ID A0A8C0PKD7
Sample Type Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples
Detection Method Sandwich ELISA, Double Antibody
Detection Wavelength OD450
Reaction Duration 4 hours
Range 3.12-200 pg/ml
Sensitivity 1.875 pg/ml
Storage 2-8°C (Sealed). Don't cryopreserve.
Specificity Specifically binds with RETN, no obvious cross reaction with other analogues.
Intended Use For research use only.

Resistin, encoded by the RETN gene, is a cysteine-rich secretory protein that plays a pivotal role in various physiological and pathological processes, particularly in metabolic regulation, inflammation, and immune responses. Initially identified as an adipose tissue-specific secretory factor (ADSF) in rodents, subsequent research has expanded its known functions and distribution across different species, including canines.
In canines, RETN (Resistin) has been linked to metabolic disorders such as obesity, insulin resistance, and inflammatory conditions, making it a critical target for veterinary and biomedical research. Studies have shown that canine Resistin levels may correlate with adipose tissue accumulation, systemic inflammation, and the progression of metabolic diseases, highlighting its significance as a research biomarker.
The demand for reliable tools to detect canine Resistin has grown with the increasing focus on understanding canine metabolic health, veterinary research, and preclinical studies. ELISA (Enzyme-Linked Immunosorbent Assay) kits are widely preferred for their high sensitivity, specificity, and ease of use in laboratory settings, enabling accurate quantification of Resistin in various biological samples to support research objectives.

Species-specific design: Tailored exclusively for canine samples, ensuring optimal binding affinity with canine Resistin and minimizing cross-reactivity with other proteins.
Versatile sample compatibility: Compatible with multiple sample types commonly used in research, including serum, plasma, cell culture supernatant, and cell or tissue lysate, providing flexibility for different experimental setups.
Sandwich ELISA technology: Utilizes a double-antibody sandwich method, a gold standard in immunoassay techniques, to ensure high specificity and reliable detection of target analytes.
Precise detection wavelength: Optimized for OD450 nm, the standard wavelength for ELISA readouts, ensuring compatibility with most laboratory microplate readers.
Well-defined detection range: Covers 3.12-200 pg/ml, allowing for the quantification of both low and high levels of canine Resistin in samples, reducing the need for sample dilution or concentration.
Exceptional sensitivity: Detects as low as 1.875 pg/ml, enabling the detection of trace amounts of Resistin, which is crucial for early-stage research or samples with low analyte concentrations.
Convenient storage conditions: Requires storage at 2-8°C (sealed) without the need for cryopreservation, simplifying inventory management and reducing the risk of reagent degradation.
Research-focused utility: Designed specifically for research applications, providing consistent and reproducible results to support data integrity in academic, veterinary, and preclinical research projects.

High specificity: Undergoes rigorous validation to ensure specific binding with canine Resistin, with no obvious cross-reactivity with other analogues or non-target proteins, guaranteeing accurate and reliable results.
Superior sensitivity: Outperforms many conventional detection methods with a low limit of detection (1.875 pg/ml), enabling researchers to measure subtle changes in Resistin levels that may be critical for their studies.
Efficient assay workflow: Features a reasonable reaction duration of 4 hours, balancing assay performance with experimental efficiency and allowing for timely completion of experiments.
Reproducible results: Manufactured under strict quality control standards, ensuring batch-to-batch consistency and minimizing experimental variability, which is essential for reliable data comparison across studies.
User-friendly operation: Follows a standard ELISA protocol that is easy to implement, even for researchers with limited immunoassay experience, reducing the learning curve and operational errors.
Broad applicability: Supports a wide range of research areas, including canine metabolic disease research, obesity studies, inflammation research, veterinary physiology, and preclinical drug development, catering to diverse research needs.
Cost-effective solution: Provides a high-quality, reliable tool at a competitive value, offering researchers an affordable option without compromising on performance or data quality.
Quality-assured reagents: All components of the kit are rigorously tested for stability, activity, and specificity, ensuring that researchers receive consistent, high-performance reagents for their experiments.

For research use only, not for clinical use.

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