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| Product Name | Borate Buffer, 0.1 M, pH 8.5 |
| Catalog No. | ATR-B-YJL-0183 |
| Description | An alkaline borate buffer system commonly employed in carbohydrate chemistry, glycoprotein analysis, and as an alternative to Tris-based buffers where primary amine reactivity is undesirable. |
| Intended Use | Buffering agent for reducing sugar assays, glycoprotein conjugation reactions, and capillary electrophoresis separations of carbohydrates. |
| Principle / Technology | Boric acid forms tetrahedral borate ions in alkaline solution that complex with cis-diol groups on carbohydrates, providing both buffering and selective glycan interaction. |
| Detection Method | pH electrode, borate-specific colorimetric assay |
| Sample Type | Glycoproteins, monosaccharides, oligosaccharides, polysaccharide preparations |
| Performance Range / Specifications | pH 8.3–8.7, effective buffering pH 8.0–10.0 |
| Sensitivity / LOD | N/A |
| Specificity | Complexation with vicinal diols provides discrimination between glycosylated and non-glycosylated species |
| Reaction Conditions / Protocol | Use at 10–100 mM working concentration; prepare fresh for long-term labeling reactions. |
| Components / Formulation | Boric acid, sodium tetraborate in ultrapure water, pH adjusted with NaOH or HCl |
| Storage Conditions | 15–30°C |
| Shelf Life | 12 months; discard if visible precipitation appears |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Quality Control | pH verification, borate concentration confirmation by titration |
| Key Features | Selective carbohydrate interaction; amine free; compatible with fluorescence labeling; standard for glycan analysis |
For research use only, not for clinical use.
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