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| Product Name | Blood Glucose Content Assay Kit, 100T/96S |
| Catalog No. | SCAK-YJL-0220 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Glucose is the primary monosaccharide circulating in the blood of mammals and serves as the most critical energy source for cells, tissues, and organs throughout the body. The maintenance of stable blood glucose levels is tightly regulated by a complex interplay of the nervous system, endocrine hormones (such as insulin and glucagon), and metabolic organs (including the liver, pancreas, and kidneys). When this regulatory balance is disrupted, it can lead to metabolic disorders like diabetes, hypoglycemia, or hyperglycemia—conditions that not only affect individual health but also require precise monitoring for research into disease mechanisms, drug development, and metabolic pathway studies.
In research settings, the accurate quantification of glucose in biological samples is fundamental to understanding various physiological and pathological processes. For example, in studies focused on diabetes, researchers need reliable glucose measurements to evaluate the efficacy of potential therapeutic agents; in cell biology, monitoring glucose consumption in cell culture supernatants helps assess cellular bioenergetics and metabolic activity; and in food science research, glucose content analysis is essential for evaluating nutritional profiles of food products.
Traditional methods for glucose detection often faced limitations such as low specificity (prone to interference from other sugars), poor sensitivity (unable to detect low-concentration glucose), or complex operational procedures (requiring extensive sample pretreatment). With the advancement of enzymatic assay technology, glucose detection kits leveraging highly specific enzymes (such as glucose oxidase, GOD) have become the gold standard in research. These kits address the shortcomings of traditional methods, enabling rapid, accurate, and high-throughput glucose quantification—making them indispensable tools for researchers in fields like molecular biology, pharmacology, food science, and clinical research (for non-diagnostic research purposes only).
Our Blood Glucose Content Assay Kit (Cat.No: SCAK-YJL-0220) is developed based on the optimized glucose oxidase-peroxidase (GOD-POD) enzymatic reaction system. It is designed to meet the diverse needs of research laboratories, supporting the quantitative determination of glucose in a wide range of samples while ensuring consistency, reliability, and ease of use.
Versatile Sample Compatibility: This kit is suitable for the quantitative determination of glucose in multiple types of biological and food samples, including serum, plasma, urine, cell culture supernatants, and food products—eliminating the need for separate kits for different sample types and simplifying experimental workflows.
Dual Detection Instrument Support: It is compatible with both spectrophotometers and microplate readers, adapting to different laboratory equipment setups. The microplate reader compatibility further enables high-throughput analysis, allowing researchers to process up to 96 samples simultaneously (matching the 100T/96S format) and significantly reducing assay time.
Stable Storage and Long Shelf Life: The kit can be stored at 2-8°C (a standard refrigerator temperature, no need for ultra-low temperature freezers) and maintains its efficacy for 6 months under proper storage conditions. This ensures long-term usability and reduces the need for frequent reordering.
Ready-to-Use Reagent Configuration: The kit includes pre-formulated reagents that require minimal preparation before use. This not only saves time spent on reagent mixing and calibration but also minimizes human error during reagent preparation, ensuring consistent results across different experiments and operators.
High Specificity for Glucose: The kit utilizes the GOD-POD enzymatic reaction system, where glucose oxidase (GOD) specifically catalyzes the oxidation of glucose (without reacting with other common sugars like fructose or galactose). This high specificity ensures that the detected signal accurately reflects glucose concentration, avoiding false positives or inaccurate results caused by sample matrix interference.
Excellent Sensitivity for Low-Concentration Detection: It can detect low concentrations of glucose with a clear, measurable absorbance signal. This advantage is particularly critical for research samples with low glucose levels (such as certain cell culture supernatants or diluted biological fluids), ensuring that even trace amounts of glucose are not missed.
Simplified and Rapid Assay Protocol: The experimental procedure is straightforward and optimized for efficiency. From sample preparation to result reading, the entire assay can be completed in a short time (depending on the number of samples, typically within 1-2 hours), reducing the waiting time for results and accelerating research progress.
Reliable Result Reproducibility: The kit’s pre-calibrated reagent system and standardized protocol ensure low inter-assay and intra-assay variation. This means that the same sample, when tested multiple times or by different operators, will yield consistent results—enhancing the credibility of research data and supporting the validity of experimental conclusions.
Cost-Effective Test Format: The 100T/96S format provides a cost-effective solution for both small-scale and medium-scale research projects. It offers enough test capacity to meet routine experimental needs while avoiding waste associated with larger-format kits that may expire before use.
For research use only, not for clinical use.
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