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| Product Name | Blocking Buffer, BSA-Based, 1% Solution |
| Catalog No. | ATR-B-YJL-0170 |
| Description | A bovine serum albumin-based blocking solution formulated to reduce non-specific protein binding in immunoassays, Western blotting, and ELISA procedures. |
| Intended Use | Reducing background signal in plate-based immunoassays, membrane-based protein detection, and immunohistochemical staining by saturating non-specific binding sites. |
| Principle / Technology | High concentration of BSA occupies uncoated surfaces, preventing non-specific adsorption of detection antibodies and reporter conjugates. |
| Detection Method | ELISA optical density measurement, Western blot background evaluation |
| Sample Type | ELISA plates, nitrocellulose and PVDF membranes, tissue sections |
| Performance Range / Specifications | Background reduction >90% compared to unblocked controls |
| Sensitivity / LOD | N/A |
| Specificity | Broad blocking of hydrophobic and non-specific protein binding |
| Reaction Conditions / Protocol | Apply to wells or membrane for 1 hour at room temperature or overnight at 4°C; wash after blocking. |
| Components / Formulation | Bovine serum albumin (Fraction V, IgG-free) at 1% w/v in PBS with 0.05% sodium azide as preservative |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months after opening |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Protein concentration verification, IgG contamination <0.1 ng/mL, lot-to-lot ELISA background comparison |
| Key Features | IgG-free BSA source; ready to use; preservative included for extended shelf life; validated for ELISA and Western blot |
For research use only, not for clinical use.
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