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| Product Name | Bis-tris propane buffer for diagnostic immunoassays, CAS 64431-96-5, pKa 9.0 |
| Catalog No. | ATR-B-YJL-0207 |
| Description | BIS-TRIS propane, chemically known as 1,3-bis[tris(hydroxymethyl)methylamino]propane, is a white crystalline powder with a pKa of 9.0 at 25 °C and a buffering range of pH 6.3-9.5. It is widely used in biochemical research, diagnostics, and pharmaceutical applications to stabilize solution pH and preserve the biological activity of proteins, enzymes, and other biomacromolecules. In biochemical workflows, it offers useful buffering capacity with minimal disruption to biomolecular structure. In diagnostic applications, it is used in immunoassays such as ELISA and Western blotting to maintain appropriate reaction conditions. It is also suitable for buffer exchange and for the purification of proteins and antibodies. |
| Intended Use | For use as a biological buffer in biochemical research, diagnostic immunoassays, pharmaceutical applications, buffer exchange, and protein or antibody purification. |
| Principle / Technology | With a pKa of 9.0 at 25 °C, BIS-TRIS propane provides buffering across pH 6.3-9.5, helping maintain stable reaction conditions and preserve the activity of proteins, enzymes, and other biomacromolecules. |
| Package Specifications | 25 kg per drum |
| Product Form | Crystalline powder |
| Key Features | Buffers across pH 6.3-9.5 Preserves the activity of proteins, enzymes, and other biomacromolecules Minimal interference with biomolecular structure Suitable for ELISA and Western blotting Applicable to buffer exchange and protein or antibody purification High solubility and stability in water and other solvents |
| Purity | >99% |
| Molecular Weight | 282.33 g/mol |
| pH Range / Optimal pH | 6.3-9.5 |
| Compatibility | Compatible with many assays and laboratory techniques. |
| Molecular Formula | C11H26N2O6 |
| Appearance | White crystalline powder |
| Alias | BIS-TRIS propane; 1,3-Bis[tris(hydroxymethyl)methylamino]propane |
| Solubility | Highly soluble in water and other solvents |
| CAS Number | 64431-96-5 |
| pKa | 9.0 at 25 °C |
| Melting Point | 164-165 °C (lit.) |
For research use only, not for clinical use.
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