Plasmid Extraction Kit (100 rxns)

Plasmid DNA extraction is a foundational technique in molecular biology, serving as a critical step for downstream applications such as gene cloning, DNA sequencing, in vitro transcription/translation, bacterial transformation, and cell transfection. As research demands for efficiency, purity, and scalability grow, traditional methods like high-speed centrifugation or vacuum filtration have gradually been replaced by advanced kit-based solutions—our Plasmid Extraction Kit (100 rxns) is developed to address these evolving needs.
This kit is designed specifically for isolating plasmid DNA from E. coli overnight cultures (1–50 mL volume range), a common host strain for plasmid propagation in laboratories. It leverages an improved SDS-based lysis method combined with dual magnetic bead extraction technology: the SDS lysis efficiently breaks down bacterial cell membranes and releases nucleic acids, while the dual magnetic beads enable specific binding of plasmid DNA and rapid removal of impurities (e.g., genomic DNA, proteins, RNA, cell debris) without relying on cumbersome high-speed centrifugation or vacuum filtration.
Importantly, our Plasmid Extraction Kit is intended for research purposes only, not for clinical use (e.g., diagnosis, prevention, or treatment of diseases). It aligns with standard molecular biology research workflows, providing a reliable tool for academic laboratories, biotech companies, and research institutions to obtain high-quality plasmid DNA for experimental use.