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| Product Name | ATP Citrate Lyase (ACL) Activity Assay Kit, 100T/96S |
| Catalog No. | FAMAK-YJL-0061 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
ATP Citrate Lyase (ACL) is a key cytoplasmic enzyme that plays a critical role in intermediary metabolism, particularly in fatty acid and cholesterol biosynthesis. It catalyzes the ATP-dependent cleavage of citrate into acetyl-CoA and oxaloacetate, providing the essential acetyl-CoA precursor for de novo lipid synthesis and serving as a link between carbohydrate metabolism and lipid biosynthesis.
In research contexts, ACL activity is widely studied due to its involvement in various physiological and pathological processes. Abnormal ACL expression or activity has been associated with metabolic disorders such as obesity, diabetes, and non-alcoholic fatty liver disease, as well as cancer progression—making it a valuable target for mechanistic research and drug discovery.
Accurate measurement of ACL activity is fundamental for understanding its biological functions and regulatory mechanisms in cellular systems. Research-grade ACL activity assay kits are designed to enable reliable quantification of enzyme activity in various biological samples, supporting studies in biochemistry, molecular biology, pharmacology, and translational research.
This ATP Citrate Lyase (ACL) Activity Assay Kit is developed specifically for research use, providing a standardized and efficient method to measure ACL activity in samples such as cell lysates, tissue homogenates, and purified enzyme preparations, without clinical application purposes.
Utilizes a micro-volume detection method compatible with spectrophotometers and microplate readers, ensuring flexibility for different laboratory setups and high-throughput sample analysis.
Offers high sensitivity and specificity, with minimal interference from other cellular components, enabling accurate and reliable quantification of ACL activity in complex biological samples.
Pre-configured reagents and optimized assay protocols reduce experimental complexity, allowing for straightforward operation and consistent results across experiments.
Stable reagent formulation with a 6-month storage period at -20°C, ensuring long-term usability and reducing the need for frequent reagent replacement.
Designed for research use with broad applicability, supporting studies in cell biology, metabolic research, cancer biology, and drug development.
Delivers precise and reproducible results, with low intra-assay and inter-assay variability, ensuring data reliability for publication-quality research.
Time-efficient assay workflow, minimizing hands-on time and enabling researchers to process multiple samples simultaneously, improving experimental productivity.
User-friendly design suitable for both experienced researchers and laboratory technicians, requiring no specialized equipment beyond standard spectrophotometers or microplate readers.
Cost-effective solution for ACL activity detection, providing sufficient reagents for 100 tests (100T) or 96-well plate-based analysis (96S), catering to different experimental scales.
Complies with research-use standards, with clear operational guidelines and safety recommendations to ensure experimental safety and compliance with laboratory protocols.
For research use only, not for clinical use.
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