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| Product Name | Antigen Retrieval Buffer, Tris-EDTA, pH 9.0, 10x |
| Catalog No. | ATR-B-YJL-0187 |
| Description | A ten-fold concentrated high-pH Tris-EDTA antigen retrieval solution for heat-induced epitope retrieval in formalin-fixed paraffin-embedded tissue immunohistochemistry. |
| Intended Use | Reversing protein cross-links in FFPE tissue sections to expose antibody epitopes masked by formalin fixation, enabling successful immunohistochemical detection. |
| Principle / Technology | Alkaline pH and elevated temperature hydrolyze methylene bridges formed during formalin fixation; EDTA chelates calcium ions that stabilize protein cross-links. |
| Detection Method | IHC staining intensity and pattern comparison with validated positive control tissues |
| Sample Type | FFPE tissue sections on charged microscope slides |
| Performance Range / Specifications | Epitope retrieval for >95% of antibodies tested that require HIER |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:10; heat in pressure cooker or microwave to 95–100°C; incubate slides for 15–30 minutes; cool for 20 minutes before continuing IHC protocol. |
| Components / Formulation | Tris base 100 mM, EDTA 10 mM, 0.05% Tween 20 at 10x, pH 9.0 |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 250 mL, 500 mL |
| Product Form | Liquid concentrate (10x) |
| Quality Control | pH verification, tissue section integrity test, positive control IHC validation |
| Key Features | High pH for difficult epitopes; tissue-safe formulation; works with pressure cooker, microwave, or water bath; standard in clinical IHC labs |
For research use only, not for clinical use.
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