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| Product Name | Annexin V-PE/7-AAD Apoptosis Detection Kit |
| Catalog No. | CAAK-0002 |
| Description | A dual-color flow-cytometry kit combining PE-labeled Annexin V with 7-AAD to distinguish viable, early-apoptotic and late-apoptotic or necrotic cell populations. |
| Intended Use | Apoptosis detection in adherent and suspension cultured cells. |
| Principle / Technology | Annexin V-PE detects phosphatidylserine externalization during early apoptosis, while 7-AAD enters cells with compromised membranes and labels DNA. |
| Detection Method | Flow cytometry. |
| Sample Type | Adherent and suspension cultured cells. |
| Performance Range / Specifications | Available for 50 or 100 reactions. |
| Specificity | Competitive blocking with unlabeled Annexin V confirms specific binding to exposed phosphatidylserine. |
| Reaction Conditions / Protocol | Stain cells with Annexin V-PE and 7-AAD and analyze by flow cytometry using appropriate single-stain and negative controls. |
| Components / Formulation | Annexin V-PE and 7-AAD staining reagents. |
| Storage Conditions | Store all components at 2-8°C. Protect Annexin V-PE and 7-AAD from light. Do not freeze. |
| Package Specifications | 50 reactions; 100 reactions. |
| Product Form | Dual-color flow-cytometry apoptosis kit. |
| Key Features | Specific dual staining; differentiates early and late apoptosis; suitable for adherent and suspension cells. |
| Compatibility | Standard flow-cytometry platforms supporting PE and 7-AAD channels. |
| Application Notes / Precautions | Use appropriate unstained and single-color controls and protect fluorescent reagents from light. |
For research use only, not for clinical use.
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