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Annexin V-mCherry/SYTOX Green Apoptosis Assay Kit

Cat.No: REKI-0031 Datasheet

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Product Name Annexin V-mCherry/SYTOX Green Apoptosis Assay Kit
Catalog No. REKI-0031
Description Two-color apoptosis detection kit pairing an mCherry-fused Annexin V with the SYTOX Green nuclear dye, offered in 20-test and 50-test formats. The red Annexin V-mCherry signal reports phosphatidylserine exposure on early apoptotic cells, while the green SYTOX signal marks cells whose membranes have lost integrity, so apoptosis staging can be separated from secondary necrosis. Store at -20 °C; the kit keeps for 12 months.
Intended Use Two-channel detection of apoptosis and membrane integrity loss
Principle / Technology Annexin V-mCherry binds externalized phosphatidylserine in a calcium-dependent manner and SYTOX Green enters only membrane-compromised cells
Specificity 20 T / 50 T; red and green fluorescence channels; 12 month shelf life
Reaction Conditions / Protocol Stain the cells with the Annexin V-mCherry and SYTOX Green working solution in binding buffer and analyze by fluorescence microscopy or flow cytometry
Storage Conditions -20 °C
Package Specifications 20 T / 50 T
Key Features Red-green combination avoids the standard FITC channel; separates apoptosis from necrosis; 12 month shelf life

For research use only, not for clinical use.

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