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Annexin V-FITC Apoptosis Assay Kit

Cat.No: REKI-0030 Datasheet

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Product Details Related Products
Product Name Annexin V-FITC Apoptosis Assay Kit
Catalog No. REKI-0030
Description Classical Annexin V apoptosis kit with the detection protein conjugated to FITC, supplied in 20-test, 50-test and 100-test sizes. FITC-labeled Annexin V binds phosphatidylserine exposed on the surface of early apoptotic cells, and the green fluorescent signal is acquired on a flow cytometer or fluorescence microscope; pairing with a membrane-impermeant dye in the laboratory adds viability staging when needed. Components store refrigerated at 2-8 °C and keep for 12 months.
Intended Use Flow cytometric or microscopic detection of early apoptotic cells by PS externalization
Principle / Technology FITC-conjugated Annexin V binds phosphatidylserine translocated to the cell surface during early apoptosis in a calcium-dependent manner
Specificity 20 T / 50 T / 100 T; green fluorescence (FITC) readout; 12 month shelf life
Reaction Conditions / Protocol Combine the cells with Annexin V-FITC in binding buffer, incubate protected from light and acquire the green fluorescence signal
Storage Conditions 2-8 °C
Package Specifications 20 T / 50 T / 100 T
Key Features Standard FITC channel compatibility; three pack sizes for different throughput; 12 month refrigerated shelf life

For research use only, not for clinical use.

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