- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Annexin V-EGFP/PI Apoptosis Detection Kit |
| Catalog No. | RESK-0141 |
| Description | This kit uses EGFP-labeled Annexin V as a probe to detect the onset of early apoptosis. In viable cells, phosphatidylserine (PS) is localized on the inner leaflet of the plasma membrane. During early apoptosis, PS is translocated from the inner to the outer leaflet and becomes exposed to the extracellular environment. Annexin V is a Ca2+-dependent phospholipid-binding protein with a molecular weight of 35-36 kDa that binds PS with high affinity, allowing fluorescently labeled Annexin V to bind to the membrane of early apoptotic cells via the exposed PS. Propidium iodide (PI) is a nucleic acid dye that cannot penetrate the intact membrane of viable or early apoptotic cells, but can enter late apoptotic and necrotic cells and stain the nucleus red. When Annexin V and PI are used in combination, PI is excluded from viable cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells are co-stained and appear double positive (Annexin V+/PI+). The kit is suitable for detection by flow cytometry and fluorescence microscopy. |
| Intended Use | For the detection and discrimination of viable, early apoptotic, late apoptotic and necrotic cells. For research use only. |
| Principle / Technology | In viable cells, phosphatidylserine (PS) is localized on the inner leaflet of the plasma membrane. During early apoptosis, PS is translocated from the inner to the outer leaflet and becomes exposed to the extracellular environment. Annexin V is a Ca2+-dependent phospholipid-binding protein with a molecular weight of 35-36 kDa that binds PS with high affinity, allowing fluorescently labeled Annexin V to bind to the membrane of early apoptotic cells via the exposed PS. Propidium iodide (PI) is a nucleic acid dye that cannot penetrate the intact membrane of viable or early apoptotic cells, but can enter late apoptotic and necrotic cells and stain the nucleus red. When Annexin V and PI are used in combination, PI is excluded from viable cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells are co-stained and appear double positive (Annexin V+/PI+). |
| Detection Method | Flow cytometry or fluorescence microscopy (EGFP green fluorescence channel for Annexin V; PI channel for membrane-compromised cells) |
| Sample Type | Cultured cells (adherent or suspension) |
| Performance Range / Specifications | Convenient and accurate detection |
| Specificity | Annexin V binds externalized phosphatidylserine in a Ca2+-dependent manner with high affinity; PI is excluded by intact membranes and stains only late apoptotic/necrotic cells |
| Reaction Conditions / Protocol | Stain cells with Annexin V-EGFP and PI in 1x Binding Buffer according to the kit manual, then analyze by flow cytometry or fluorescence microscopy. Viable cells (Annexin V-/PI-); early apoptotic cells (Annexin V+/PI-); late apoptotic/necrotic cells (Annexin V+/PI+). |
| Components / Formulation | Annexin V-EGFP conjugate Propidium Iodide (PI) Binding Buffer (Ca2+-containing) |
| Storage Conditions | Store at -20°C protected from light; avoid repeated freeze-thaw cycles. For repeated use within a short period, store at 4°C protected from light. |
| Shelf Life | 2 years when stored at -20°C protected from light; 6 months when stored at 4°C protected from light |
| Package Specifications | 20 T 50 T 100 T |
| Product Form | Liquid kit (ready-to-use fluorescent probes and buffer) |
| Molecular Weight | Annexin V: 35-36 kDa |
| Shipping Conditions | Shipped on wet ice |
| Expiration Date / Stability | Stable for 2 years at -20°C protected from light (avoid repeated freeze-thaw); stable for 6 months at 4°C protected from light |
| Regulatory / Compliance | For research use only |
| Compatibility | Flow cytometers and fluorescence microscopes with GFP/EGFP (Ex/Em approximately 488/507 nm) and PI detection channels |
| Recommended Buffer System | 1x Binding Buffer supplied in the kit |
| Application Notes / Precautions | Protect fluorescent reagents from light during handling and incubation to avoid photobleaching. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.