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Annexin V-EGFP Apoptosis Assay Kit

Cat.No: REKI-0029 Datasheet

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Product Name Annexin V-EGFP Apoptosis Assay Kit
Catalog No. REKI-0029
Description Apoptosis detection kit in which Annexin V is fused to enhanced green fluorescent protein, reporting phosphatidylserine externalization in the green channel. Early apoptotic cells bind the labeled protein in a calcium-dependent manner while their plasma membranes remain intact, which is the hallmark event this kit captures. Offered in 20-test and 50-test formats with refrigerated storage; the components keep for 12 months at 2-8 °C.
Intended Use Detection of early apoptosis through PS externalization in the green fluorescence channel
Principle / Technology Annexin V-EGFP binds exposed phosphatidylserine on the outer membrane leaflet of early apoptotic cells in a calcium-dependent reaction
Specificity 20 T / 50 T; green fluorescence channel readout; 12 month shelf life
Reaction Conditions / Protocol Resuspend the cells in binding buffer with the Annexin V-EGFP reagent, incubate and analyze by flow cytometry or fluorescence microscopy
Storage Conditions 2-8 °C
Package Specifications 20 T / 50 T
Key Features EGFP reporter for standard FL1 acquisition; early-apoptosis specific readout; 12 month refrigerated shelf life

For research use only, not for clinical use.

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