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| Product Name | Annexin V-APC/PI Apoptosis Detection Kit |
| Catalog No. | CAAK-0001 |
| Description | A dual-fluorescence apoptosis assay using APC-labeled Annexin V and propidium iodide to classify viable, early-apoptotic and late-apoptotic or necrotic cells. |
| Intended Use | Quantitative apoptosis detection in adherent and suspension cell cultures. |
| Principle / Technology | APC-labeled Annexin V binds phosphatidylserine exposed on early-apoptotic cells, while propidium iodide enters membrane-compromised late-apoptotic or necrotic cells and binds DNA. |
| Detection Method | Flow cytometry or fluorescence microscopy. |
| Sample Type | Adherent and suspension cultured cells. |
| Performance Range / Specifications | Staining can be completed in approximately 15-20 minutes; available for 50 or 100 reactions. |
| Specificity | Dual staining differentiates viable, early-apoptotic and late-apoptotic or necrotic populations. |
| Reaction Conditions / Protocol | Stain harvested cells with Annexin V-APC and propidium iodide, protect from light and analyze promptly. |
| Components / Formulation | Annexin V-APC and propidium iodide staining reagents. |
| Storage Conditions | Store all components at 2-8°C. Protect Annexin V-APC and propidium iodide from light. Do not freeze. |
| Package Specifications | 50 reactions; 100 reactions. |
| Product Form | Fluorescent apoptosis assay kit. |
| Key Features | Clear population separation; 15-20 minute staining; compatible with GFP-expressing cells; suitable for adherent and suspension cultures. |
| Compatibility | Flow cytometers and fluorescence microscopes; GFP fluorescence does not interfere with the APC/PI combination. |
| Application Notes / Precautions | Protect fluorescent reagents from light and analyze samples promptly after staining. |
For research use only, not for clinical use.
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