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| Product Name | β-Amylase (β-AL) Activity Assay Kit (Iodine-starch Colorimetry), 100T/48S |
| Catalog No. | SCAK-YJL-0051 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | β-Amylase is an exo-acting enzyme that hydrolyzes α-1,4-glycosidic bonds from the non-reducing ends of starch and glycogen, sequentially releasing maltose units. It is particularly important in plants (e.g., germinating seeds) and is a key enzyme in the malting and brewing industries. Unlike α-amylase, it cannot bypass α-1,6 branch points. This kit specifically measures β-amylase activity by quantifying the decrease in the starch-iodine complex as the enzyme cleaves maltose from the starch polymer. |
| Product Application | • Research on starch degradation during seed germination and plant development. • Quality control in malting and brewing processes. • Characterization of microbial or plant-derived enzyme preparations. • Studies comparing the action patterns of different amylolytic enzymes. |
| Product Advantages | • Specific for β-Amylase Activity: The assay conditions and interpretation are optimized to reflect the exo-acting mechanism of β-amylase, distinguishing it from endo-acting α-amylase activity when used appropriately with controlled substrates. • Classic, Visual Method: Utilizes the well-established iodine-starch reaction, providing a clear color change from blue to reddish-brown that is easy to monitor and quantify spectrophotometrically. • Cost-Effective & Reliable: Based on stable, inexpensive chemicals, offering a robust and economical solution for routine β-amylase measurement in various sample types. • Defined Substrate: Includes a purified starch substrate with consistent chain length and branching, ensuring reproducible enzyme kinetics and reliable comparison between experiments. |
For research use only, not for clinical use.
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