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| Product Name | ALD (Aldosterone) ELISA Kit |
| Catalog No. | HTK-YJL-0466 |
| Synonyms | ALD ELISA Kit, Aldosterone ELISA Kit |
| Species | Universal |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 3.125-200 pg/ml |
| Sensitivity | 1.875 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with ALD, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Aldosterone (ALD) is a steroid hormone primarily synthesized and secreted by the zona glomerulosa of the adrenal cortex. It plays a pivotal role in maintaining the body’s electrolyte balance and regulating arterial blood pressure, making it a critical target for research in endocrinology, metabolic pathways, and cardiovascular physiology.
The regulation of ALD secretion is mainly controlled by the renin-angiotensin-aldosterone system (RAAS). When the body experiences reduced renal perfusion pressure, decreased plasma volume, or negative sodium balance, renin production is stimulated. Renin then cleaves angiotensinogen to form angiotensin I, which is further converted to angiotensin II by angiotensin-converting enzyme (ACE). Angiotensin II not only causes vascular constriction but also directly stimulates the adrenal cortex to secrete ALD.
Functionally, ALD acts on mineralocorticoid receptors in the distal convoluted tubules and collecting ducts of the nephron. It promotes the reabsorption of sodium (Na⁺) and chloride (Cl⁻) by the kidneys, while enhancing the excretion of potassium (K⁺) and hydrogen ions (H⁺). This process increases water retention, expands plasma volume, and ultimately elevates blood pressure—key mechanisms that support the body’s homeostatic balance.
In research contexts, accurate quantification of ALD levels is essential for studying conditions such as primary aldosteronism (PA), hypertension, edema, and electrolyte disorders. PA, a group of diseases characterized by excessive and unregulated ALD production (independent of angiotensin II and potassium levels), has a prevalence of over 5% (and up to 10%) in hypertensive populations. PA patients also face higher risks of cardiovascular events, organ damage (e.g., heart and kidney impairment), metabolic syndrome, diabetes, and osteoporotic fractures—highlighting the urgency of reliable ALD detection tools for research.
The ALD (Aldosterone) ELISA Kit is designed to address this research need by providing a sensitive, specific, and efficient method for quantifying ALD in various biological samples. It enables researchers to explore ALD’s role in physiological processes and disease mechanisms, supporting advancements in endocrinological and cardiovascular research.
Universal Species Compatibility: The kit is suitable for detecting ALD across multiple species, eliminating the need for species-specific kits and providing flexibility for diverse research models (e.g., human, mouse, rat, guinea pig samples).
Broad Sample Applicability: It can process a wide range of biological samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples—accommodating different research workflows and sample types commonly used in endocrinology and cell biology studies.
Competitive ELISA with Antigen Coating: Adopts a competitive ELISA format coated with ALD antigen, ensuring specific binding between the target antigen and detection reagents. This design minimizes non-specific cross-reactivity and enhances the accuracy of ALD quantification.
Optimal Detection Performance: Features a detection range of 3.125–200 pg/ml and a high sensitivity of 1.875 pg/ml, allowing for the precise measurement of both low and moderate ALD concentrations—critical for detecting subtle changes in ALD levels in research samples.
Time-Efficient Assay: The total reaction duration is only 2 hours, reducing experimental turnaround time and enabling researchers to process more samples within a shorter period, improving research efficiency.
Stable Storage Conditions: Can be stored at 2–8°C (sealed) without the need for cryopreservation, simplifying inventory management and reducing the risk of reagent degradation due to improper freezing/thawing cycles.
High Specificity: Specifically binds to ALD with no obvious cross-reactivity with other structural analogues, ensuring that the detected signal accurately reflects ALD concentrations and avoiding false results in research experiments.
Superior Sensitivity for Low-Level Detection: With a sensitivity of 1.875 pg/ml, the kit can detect very low concentrations of ALD that may be missed by less sensitive assays. This is particularly valuable for research on early-stage disease models or physiological states where ALD levels are only slightly altered.
Reliable Reproducibility: Based on standard ELISA validation principles (consistent with industry practices for precision testing), the kit ensures low intra-assay and inter-assay coefficient of variation (CV). This reliability guarantees that research results are consistent across repeated experiments, enhancing the credibility of study findings.
User-Friendly Operation: The kit follows a straightforward competitive ELISA protocol, and all necessary reagents (e.g., pre-coated plates, detection antibodies, substrates) are designed to work seamlessly together. This reduces the learning curve for researchers, even those with limited ELISA experience, and minimizes experimental errors.
Cost-Effective Sample Processing: Available in 48T and 96T formats, the kit allows researchers to choose the appropriate size based on sample volume—avoiding reagent waste and optimizing cost efficiency for both small-scale pilot studies and large-scale experiments.
Research-Focused Design: Strictly manufactured for research use only, the kit meets the specific needs of academic and industrial research labs (e.g., endocrinology, metabolic research, cardiovascular physiology). It aligns with standard research workflows and quality requirements, ensuring compatibility with common laboratory equipment (e.g., absorbance microplate readers at 450 nm).
For research use only, not for clinical use.
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