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| Product Name | AdvanceFast 1st Strand cDNA Synthesis Kit (No Dye) |
| Catalog No. | RESK-0021 |
| Description | The AdvanceFast 1st Strand cDNA Synthesis Kit (No Dye) is a rapid reverse transcription kit developed from the third-generation cDNA synthesis platform and is suitable for both PCR and RT-qPCR workflows. Compared with its predecessor, it delivers reliable detection rates, specificity, and yields while cutting total reverse transcription time to under 6 minutes. The kit includes a gDNA Digester Mix that eliminates residual genomic DNA contamination from RNA templates for more dependable downstream results. Both Random Primers N6 and Oligo (dT)18 are supplied, and users may also choose gene-specific primers. This version contains no tracking dye, making it the preferred option for applications where a dye tracer could interfere. The resulting single-stranded cDNA can be used directly in PCR or qPCR. |
| Intended Use | Rapid first-strand cDNA synthesis from RNA templates with genomic DNA removal, for downstream PCR amplification or RT-qPCR analysis. |
| Principle / Technology | Fast reverse transcription based on an engineered reverse transcriptase, combined with a gDNA Digester Mix that removes contaminating genomic DNA from RNA templates before cDNA synthesis. Flexible priming with Random Primers N6, Oligo (dT)18, or user-supplied gene-specific primers. Dye-free formulation without a tracking tracer. |
| Detection Method | Reverse transcription followed by PCR or qPCR (dye- or probe-based quantification). |
| Sample Type | Total RNA (e.g., 293T cell total RNA); compatible with RNA input from 10 pg to 1 μg. |
| Performance Range / Specifications | Reverse transcription completed in as little as 5 minutes (total time under 6 minutes); synthesizes cDNA fragments up to 14 kb; detection of RNA input from 10 pg to 1 μg; kit stable at 4°C for 14 days; cDNA product stable at 37°C for 7 days and through 50 freeze-thaw cycles. |
| Sensitivity / LOD | Successful reverse transcription with RNA input as low as 10 pg. |
| Specificity | Stable detection rate and specificity; 5-minute reverse transcription efficiency equal to or better than leading competing products (which require 13-20 minutes), with comparable or lower Ct values. |
| Reaction Conditions / Protocol | Standard reverse transcription is completed in 5 minutes; Ct values differ by less than 0.2 across different reverse transcription times. For direct synthesis of fragments longer than 10 kb, extend the reverse transcription time to 20-30 minutes. |
| Components / Formulation | 4x AdvanceFast SuperMix (No Dye): 50 μL (10 T) / 500 μL (100 T) 5x gDNA Digester Mix: 20 μL (10 T) / 200 μL (100 T) Random Primers (50 μM): 20 μL (10 T) / 200 μL (100 T) Oligo d(T)18 Primers (50 μM): 20 μL (10 T) / 200 μL (100 T) RNase-free Water: 200 μL (10 T) / 1 mL x 2 (100 T) |
| Storage Conditions | Store at -20°C. The cDNA product can be used immediately or stored short-term at -20°C; for long-term storage, aliquot and keep at -80°C, avoiding repeated freeze-thaw cycles. |
| Shelf Life | 12 months when stored at -20°C. |
| Package Specifications | 10 T, 100 T |
| Product Form | Kit |
| Quality Control | Kit stable at 4°C for 14 days; reverse transcription product stable at 37°C for 7 days and through 50 repeated freeze-thaw cycles. |
| Concentration | Random Primers: 50 μM; Oligo d(T)18 Primers: 50 μM |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 12 months at -20°C; cDNA product stable at 37°C for 7 days and through 50 freeze-thaw cycles. |
| Compatibility | Suitable for downstream PCR and qPCR. A dye-free version is recommended where a tracking dye may interfere with the application. |
| Application Notes / Precautions | This kit is the dye-free counterpart of the tracer-containing version; validated tests show the tracking dye in the tracer version does not affect reverse transcription efficiency, qPCR Ct values, melt curve peaks, or Tm values. Choose this No Dye version for applications where a tracking dye could cause interference. |
For research use only, not for clinical use.
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