- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Advanced One-Step RT-qPCR SYBR Green Kit |
| Catalog No. | RESK-0066 |
| Description | The Advanced One-Step RT-qPCR SYBR Green Kit performs fluorescence-based quantification using SYBR Green I dye. With gene-specific primers, reverse transcription and qPCR are completed in a single tube, eliminating repeated cap-opening and pipetting steps, greatly improving detection efficiency and reducing contamination risk. For RNA samples, a thermostable reverse transcriptase efficiently synthesizes cDNA, while an antibody-based hot-start Taq DNA polymerase drives quantitative amplification. Within its optimized buffer system, the kit achieves detection sensitivity down to 0.1 pg for highly expressed targets and 1 pg for moderately expressed targets. The kit is equally suitable for amplification and quantification of DNA samples, enabling highly sensitive detection and quantification of nucleic acids from a wide range of plant and animal samples, cells, and microorganisms. |
| Intended Use | Highly sensitive one-step RT-qPCR detection and quantification of RNA (and direct qPCR of DNA) from plant and animal samples, cells, and microorganisms. |
| Principle / Technology | One-tube reverse transcription and qPCR with gene-specific primers; thermostable reverse transcriptase for efficient cDNA synthesis combined with an antibody-based hot-start Taq DNA polymerase in an optimized buffer system. |
| Detection Method | SYBR Green I dye-based fluorescence detection. |
| Sample Type | RNA samples from plants, animals, cells, and microorganisms; also suitable for direct amplification and quantification of DNA samples. |
| Performance Range / Specifications | Universal instrument compatibility: suitable for common real-time PCR instruments of all ROX types (No ROX, Low ROX, High ROX); targets of varying GC content (30%-70%) are efficiently amplified with single, sharp melt peaks. High sensitivity: down to 0.1 pg for highly expressed RNA targets and 1 pg for moderately expressed targets; viral RNA detectable to the E2 level; DNA templates detectable down to single-copy level. Excellent repeatability: minimal variation across 90 replicate wells at high, medium, and low template inputs. High precision: accurately discriminates 2-fold differences in RNA or DNA template concentration. Excellent stability: assembled reaction mixes can stand at 4°C for 24 h without performance change; reagents show no significant difference after 20 h of ambient light exposure. Annealing temperature 60°C; amplicon length 80-300 bp. |
| Sensitivity / LOD | RNA targets: 0.1 pg (high expression), 1 pg (moderate expression); viral RNA to the E2 level. DNA targets: single-copy detection with amplification signal. |
| Specificity | Single melt peaks across targets of varying GC content (30%-70%); hot-start Taq polymerase suppresses nonspecific amplification. |
| Reaction Conditions / Protocol | Reverse transcription and qPCR are performed sequentially in the same tube; recommended annealing temperature 60°C; recommended amplicon length 80-300 bp. For DNA samples, the reverse transcription step can be skipped and qPCR performed directly. |
| Components / Formulation | 2x Advanced SG Buffer: 250 μL (20 T) / 2x1.25 mL (200 T) Advanced UH Enzyme Mix: 20 μL (20 T) / 200 μL (200 T) RNase-free H2O: 250 μL (20 T) / 2x1.25 mL (200 T) |
| Storage Conditions | Store at -25°C to -15°C, protected from light. |
| Shelf Life | 1 year when stored at -25°C to -15°C, protected from light. |
| Package Specifications | 20 T; 200 T |
| Product Form | Mix |
| Quality Control | Sensitivity, repeatability, precision, and stability verified across multiple instruments and template types; 2-fold concentration discrimination confirmed by 2-fold serial dilution of 293T cell RNA. |
| Expiration Date / Stability | 1 year at -25°C to -15°C protected from light; assembled reaction mixes stable at 4°C for 24 h. |
| Compatibility | Compatible with the vast majority of real-time PCR instruments, including No ROX and Low/High ROX platforms; universal ROX formulation. GC range 30%-70%. |
| Recommended Buffer System | 2x optimized SYBR Green buffer with dNTPs (included). |
| Application Notes / Precautions | Suitable for RNA or DNA detection from plant and animal samples, cells, and microorganisms; for RNA samples the kit efficiently synthesizes cDNA and performs quantitative amplification, while DNA samples can be amplified directly without a reverse transcription step. Store at -25°C to -15°C protected from light; valid for 1 year. Supports No ROX and Low/High ROX instruments; refer to the manual for specific models. |
| Batch-to-Batch Consistency | Minimal variation across 90 replicate wells at high, medium, and low template inputs demonstrates strong reproducibility. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
