- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Adhesion Microscope Slides, Silane-Coated, 75 x 25 mm, 1.0 mm Thick, Pre-Cleaned |
| Catalog No. | IGM-HMM-0071 |
| Description | Silane-coated (aminopropyltriethoxysilane, APTES) adhesion microscope slides providing a positively charged surface for enhanced electrostatic attachment of tissue sections, cells, and biomolecules. The aminosilane coating introduces positively charged amino groups on the glass surface at physiological pH, which electrostatically bind the negatively charged phosphate backbone of DNA/RNA, the carboxyl groups of proteins, and the negatively charged surfaces of cells and tissue sections. This eliminates the need for poly-L-lysine coating, gelatin subbing, or other manual adhesive treatments. Slides are manufactured from soda-lime glass, pre-cleaned to remove particulates, oils, and debris, and individually silane-coated in a controlled process for uniform, consistent coating thickness. Packaged in boxes of 72 with interleaving paper to prevent surface damage. |
| Intended Use | Microscope slide substrate for: immunohistochemistry (IHC) and immunocytochemistry (ICC) requiring tissue section retention through antigen retrieval (heat, enzymatic, acidic); in situ hybridization (ISH, FISH) requiring DNA/RNA probe retention through stringent wash steps; H&E and special histological staining; frozen sections (cryostat sections); cytospin preparations; and laser capture microdissection with membrane slides. |
| Principle / Technology | APTES (3-aminopropyltriethoxysilane) covalently bonds to the glass surface through silanol (-Si-OH) groups via siloxane (Si-O-Si) linkages. The exposed primary amine groups (pKa ~9-10) are protonated and positively charged at physiological and slightly acidic pH, providing strong electrostatic adhesion to negatively charged biomolecules and tissue sections. Unlike poly-L-lysine (which desorbs under heat or high salt), the covalent silane bond withstands high-temperature antigen retrieval (95-100 C), enzymatic digestion, and stringent wash conditions. |
| Detection Method | Ready-to-use silane-coated slides; no additional coating, subbing, or activation required; use directly for: mounting paraffin or frozen tissue sections (sections will adhere upon contact with the positively charged surface); baking paraffin sections at 56-60 C for 30 min to 2 h enhances adhesion; proceed with deparaffinization, antigen retrieval, and staining per standard protocols. |
| Sample Type | Formalin-fixed paraffin-embedded (FFPE) tissue sections (3-10 um), frozen tissue sections (5-20 um), cultured cells (cytospin or direct growth), cytology smears, chromosome spreads, and FISH/ISH preparations. |
| Performance Range / Specifications | Slide dimensions: 75.0 x 25.0 mm (+0/-1.0 mm); thickness: 1.0 mm (+/-0.05 mm); glass type: soda-lime (hydrolytic class 3); coating: APTES aminosilane monolayer; surface charge: positive (zeta potential +10 to +30 mV at pH 7.4); tissue adhesion: >95% section retention after antigen retrieval (citrate buffer pH 6.0, 95 C, 20 min). |
| Sensitivity / LOD | Tissue section retention on silane-coated slides: typically >98% for paraffin sections after standard processing; >95% after heat-induced epitope retrieval (HIER) at 95-100 C for 20 min; superior to poly-L-lysine coated slides for high-temperature protocols. |
| Specificity | Positively charged aminosilane surface provides non-specific electrostatic adhesion to all negatively charged biomolecules and tissues; does not selectively bind specific cell types, proteins, or nucleic acids — specificity of detection is determined entirely by downstream staining/ISH probes and antibodies. |
| Reaction Conditions / Protocol | Tissue adhesion: immediate upon contact; enhanced adhesion after baking (56-60 C, 30 min to 2 h for paraffin sections; air-dry for frozen sections); withstands antigen retrieval up to 100 C, enzymatic digestion (trypsin, proteinase K), and multiple wash steps in PBS, TBS, and SSC buffers. |
| Components / Formulation | Silane-Coated Adhesion Slides (72 slides/box), interleaved with glassine paper. |
| Storage Conditions | Store at RT (15-25 C) in original box, protected from dust and high humidity; do not store in areas with volatile organic compounds (VOCs) that may react with amino groups. |
| Shelf Life | 24 months from date of manufacture at RT in original sealed packaging; after opening box, use within 6 months for optimal adhesion performance (amino groups slowly oxidize in air). |
| Package Specifications | Box of 72 slides; case of 10 boxes (720 slides). |
| Product Form | Pre-cleaned soda-lime glass slide, 75 x 25 x 1.0 mm, frosted writing area at one end, aminosilane-coated on one side (indicated by frosted end orientation). |
| Quality Control | Each lot: surface charge (zeta potential +10 to +30 mV, pH 7.4); coating uniformity (water contact angle 45-65 degrees); tissue adhesion (>95% retention after citrate HIER, 95 C, 20 min); cleanliness (no visible particulates, residue, or staining artifacts); flatness (<10 um deviation across slide surface); DNase/RNase: not detected; dimensions within tolerance. |
| Key Features | Silane-coated (APTES) for covalent adhesion; withstands 100 C antigen retrieval; no manual coating required; positively charged surface; frosted writing area; pre-cleaned and ready-to-use; 72 slides/box. |
| Purity | Glass: soda-lime, hydrolytic class 3, free of lead and arsenic; APTES: >98% purity; coating thickness: monolayer (approximately 1-2 nm); no detectable DNase, RNase, or heavy metal contaminants. |
| Concentration | APTES coating: monomolecular layer; amino group density: 2-5 amino groups/nm^2 (theoretical: approximately 3-4/nm^2 for complete monolayer). |
| Activity / Unit Definition | Adhesion: >95% section retention after standard HIER; zeta potential: +10 to +30 mV at pH 7.4. |
| Molecular Weight | APTES: 221.37 g/mol (C9H23NO3Si); glass: SiO2 (~60%), Na2O (~15%), CaO (~10%), MgO, Al2O3. |
| Source / Origin | Glass: soda-lime float glass; APTES: synthetic organosilane; manufactured in ISO-certified facility; no animal-derived components. |
| pH Range / Optimal pH | Silane coating stable at pH 3.0-10.0 for up to 24 h at RT; antigen retrieval up to pH 9.0 (Tris-EDTA) at 95-100 C; amino groups protonated and positively charged below pH 9. |
| Shipping Conditions | Ambient temperature; protect from moisture and crushing. |
| Expiration Date / Stability | 24 months at RT in original sealed packaging; after opening: 6 months at RT (protect from humidity and airborne contaminants). |
| Regulatory / Compliance | For research use only; not for diagnostic or therapeutic use. Not for clinical diagnostic reporting without validation per local regulations. CE marked for laboratory use. |
| Compatibility | Compatible with: IHC (DAB, AP, fluorescence detection); immunofluorescence; FISH (DNA and RNA probes); H&E staining; special stains (PAS, Masson's trichrome, Congo red, etc.); in situ hybridization (chromogenic and fluorescent); laser capture microdissection; automated slide stainers (Ventana, Leica Bond, Dako, Lab Vision); manual staining protocols. Compatible with antigen retrieval methods: heat-induced (citrate pH 6.0, Tris-EDTA pH 9.0, 95-100 C, 10-40 min), enzymatic (trypsin, proteinase K, pepsin), and combined methods. Slides compatible with xylene, ethanol, and aqueous mounting media. |
| Recommended Buffer System | Silane coating stable in: PBS pH 7.4, TBS pH 7.6, SSC buffer (0.15-2x), citrate buffer pH 6.0, Tris-EDTA pH 9.0, and all standard IHC/ISH wash buffers. |
| Application Notes / Precautions | Slides are coated on one side only; the frosted writing area indicates the coated (upper) surface. Do not touch the coated surface with bare hands (oil and proteins from fingerprints reduce adhesion). Use clean forceps to handle slides by edges. For FFPE sections: mount sections on coated slides using a water bath (40-45 C), drain excess water, and bake at 56-60 C for 30 min to 2 h (or overnight at 37 C). For frozen sections: thaw-mounted sections onto RT slides and air-dry for 30-60 min before fixation. For cytospin preparations: cytospin cells directly onto coated slide, air-dry, and fix per protocol. Silane-coated slides are not recommended for applications requiring negatively charged surfaces. If background staining is observed (especially with IHC), the positive surface charge may cause non-specific antibody binding — increase blocking concentration/time or use charged slide-specific blocking protocols. |
| Batch-to-Batch Consistency | Zeta potential +10 to +30 mV for every lot; tissue retention >95% after HIER; coating uniformity verified; cleanliness and dimensions inspected. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
