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| Product Name | Acyltransferase (AAT) Activity Assay Kit, 100T/96S |
| Catalog No. | FAMAK-YJL-0024 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Kit Includes | Extraction Reagent: 100 mL×1. Storage at 4℃. Reagent I: Liquid 15 mL×1. Storage at 4℃. Reagent Ⅱ: Powder×1. Storage at -20℃. Add 1 mL of distilled water to dissolve the powder before use. The dissolved reagent is stored at 4℃. Reagent Ⅲ: Liquid 2 mL×1. Storage at 4℃. Reagent IV: Powder×1. Storage at -20℃ and protect from light. Add 1 mL of Reagent I to dissolve the powder before use. The dissolved reagent is stored at 4℃ and protect from light. |
| Product Introduction | Acyltransferases represent a large family of multifunctional proteins that play crucial roles in numerous biological processes. They primarily catalyze acylation and deacylation reactions, contributing significantly to the regulation of gene expression, metabolic pathways, and cellular signaling. In an assay for acyltransferase activity, the enzyme catalyzes the transfer of an acetyl group from acetyl-CoA to butanol. Concurrently, the reduction of DTNB (5,5′-dithiobis-(2-nitrobenzoic acid)) produces TNB (2-nitro-5-thiobenzoate), which exhibits a characteristic absorption peak at 412 nm. By monitoring the rate of increase in absorbance at this wavelength, the activity of acyltransferase can be quantitatively determined. |
| Product Application | For metabolic disease research, lipid metabolism studies, and functional analysis of enzyme activity in biological samples such as tissues, cells, and serum. |
| Product Advantages | • Simple & Convenient Protocol: The kit is designed for a straightforward workflow, allowing for quick and efficient measurement of AAT activity without the need for complex procedures. • High Reliability: Provides highly reproducible and accurate results, ensuring confidence in your data for critical research on metabolic pathways. • Ready-to-Use Reagents: Includes all necessary components optimized for the assay, saving valuable preparation time and ensuring consistent performance. • Versatile Application: Suitable for a wide range of research applications, particularly in studying lipid metabolism, enzyme kinetics, and related biochemical functions. |
For research use only, not for clinical use.
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