| Product Name | Acid Invertase (AI) Activity Assay Kit, 100T/48S |
| Catalog No. | SCAK-YJL-0143 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Characteristics | Required reagents and equipment but not provided include: spectrophotometer/microplate reader, desktop centrifuge, water-bath, adjustable pipette, micro glass cuvette/96-well flat-bottom plate, mortar/homogenizer, ice and distilled water. |
| Kit Includes | Extract solution:100 mL×1. Storage at 4℃. Reagent I: 40 mL×1. Storage at 4℃. Reagent II: Powder×1. Storage at 4℃. Add 20 mL of Reagent I to fully dissolve for standby when the solution will be used. Unused reagent is still stored at 4℃. Reagent III: 20 mL×1. Storage at 4℃. Standard solution: Powder×1. Storage at 4℃. Add 1 mL of distilled water with filly dissolve before use to prepare 10 mg/mL glucose standard solution for standby. |
| Product Introduction | Invertase (Ivr) is a crucial enzyme in sucrose metabolism of higher plants, catalyzing the irreversible hydrolysis of sucrose into fructose and glucose. Based on its optimal pH, invertase is classified into two types: acid invertase (AI) and neutral invertase (NI). Acid invertase (AI) is primarily located in vacuoles or apoplastic spaces and functions optimally under acidic conditions (pH 4.5–5.0). It plays a key role in regulating sucrose utilization within vacuoles and influences sugar accumulation during fruit development. The activity of AI can be quantified through a colorimetric method: the enzyme catalyzes the breakdown of sucrose into reducing sugars, which then react with 3,5-dinitrosalicylic acid (DNS) to form a reddish-brown product. This compound exhibits a characteristic absorption peak at 540 nm. Within a defined concentration range, the rate of increase in absorbance at 540 nm is directly proportional to AI activity, allowing accurate enzymatic assessment. |
| Product Application | For accurate measurement of acid invertase activity in plant tissues, microbial cultures, and other biological samples to support sugar metabolism and agricultural research. |
| Product Advantages | • High Sensitivity: Enables detection of low enzyme activity levels with excellent precision and linearity • Specific Substrate Recognition: Utilizes sucrose-specific reaction system ensuring accurate measurement of invertase activity • Simple Protocol: Features straightforward procedure with minimal processing steps, reducing operational time • Broad Sample Compatibility: Validated for plant tissues, fruits, microbial samples, and other biological materials • Reproducible Results: Delivers consistent performance with low batch-to-batch variation |
For research use only, not for clinical use.
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