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647-EdU Cell Proliferation Assay Kit

Cat.No: CAAK-0008 Datasheet

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Product Name 647-EdU Cell Proliferation Assay Kit
Catalog No. CAAK-0008
Description A far-red EdU proliferation assay for detecting newly synthesized DNA at single-cell resolution using a 647-channel fluorescent azide probe.
Intended Use Cell proliferation, cell-viability and cytotoxicity studies.
Principle / Technology EdU incorporated into replicating DNA is detected by a copper-catalyzed covalent click reaction with a far-red fluorescent azide probe.
Detection Method Fluorescence microscopy and flow cytometry.
Sample Type Proliferating cultured cells.
Performance Range / Specifications 100-reaction format with 647-channel fluorescent detection.
Specificity Strong far-red fluorescence and low background support accurate identification of proliferating cells.
Reaction Conditions / Protocol Pulse cells with EdU, fix and permeabilize, perform the fluorescent click reaction, wash and analyze by imaging or flow cytometry.
Components / Formulation EdU labeling reagent and 647-channel fluorescent azide click-reaction components.
Storage Conditions Store at -30 to -15°C protected from light.
Package Specifications 100 reactions.
Product Form Far-red fluorescent EdU cell-proliferation kit.
Key Features Single-cell proliferation tracing; strong far-red fluorescence; low background; imaging and flow-cytometry compatibility.
Shipping Conditions Ship at 0°C or below.
Compatibility Fluorescence imaging systems and flow cytometers with a suitable 647-channel configuration.
Application Notes / Precautions Protect fluorescent reagents from light and wash samples thoroughly after labeling.

For research use only, not for clinical use.

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