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| Product Name | 594-EdU Cell Proliferation Assay Kit |
| Catalog No. | CAAK-0007 |
| Description | A fluorescence-based EdU assay for tracing DNA synthesis and cell proliferation at single-cell resolution using a 594-channel fluorescent azide probe. |
| Intended Use | Cell proliferation, cell-viability and cytotoxicity studies. |
| Principle / Technology | EdU is incorporated into replicating DNA in place of thymidine and detected through a copper-catalyzed covalent click reaction with a fluorescent azide probe. |
| Detection Method | Fluorescence microscopy and flow cytometry. |
| Sample Type | Proliferating cultured cells. |
| Performance Range / Specifications | 100-reaction format with 594-channel fluorescent detection. |
| Specificity | Strong fluorescence with low staining background supports identification of proliferating cells. |
| Reaction Conditions / Protocol | Pulse cells with EdU, fix and permeabilize, perform the fluorescent click-labeling reaction, wash and analyze by imaging or flow cytometry. |
| Components / Formulation | EdU labeling reagent and 594-channel fluorescent azide click-reaction components. |
| Storage Conditions | Store at -30 to -15°C protected from light. |
| Package Specifications | 100 reactions. |
| Product Form | Fluorescent EdU cell-proliferation kit. |
| Key Features | Single-cell proliferation tracing; strong fluorescence; low background; compatible with imaging and flow cytometry. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Fluorescence imaging systems and flow cytometers with a suitable 594-channel configuration. |
| Application Notes / Precautions | Protect fluorescent reagents from light and wash samples thoroughly after the click-labeling reaction. |
For research use only, not for clinical use.
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