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| Product Name | 5× PCR Enhancer |
| Catalog No. | ATR-B-YJL-0202 |
| Description | A multi-component 5× PCR additive formulated to improve amplification stability and efficiency for high-GC templates and DNA with strong secondary structures. |
| Intended Use | Optimization of difficult PCR systems, especially high-GC and structurally complex templates. |
| Principle / Technology | The additive lowers the effective melting temperature of high-GC DNA and destabilizes template secondary structures to improve polymerase access. |
| Detection Method | Used as an additive in endpoint PCR followed by electrophoretic analysis. |
| Sample Type | High-GC DNA and templates with stable secondary structures. |
| Performance Range / Specifications | Demonstrated with 690 bp targets containing 72% GC. |
| Reaction Conditions / Protocol | For a 50 μL PCR, add 10 μL of the 5× enhancer for a 1× final concentration. For high-fidelity PCR, use a 0.5–1× final concentration. |
| Components / Formulation | Multi-component PCR-enhancing additive. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 500 μL; 5 × 500 μL. |
| Product Form | 5× PCR additive solution. |
| Key Features | Improves amplification stability and efficiency; supports high-GC templates; helps resolve complex secondary structures. |
| Concentration | 5×. |
| Compatibility | Compatible with routine PCR systems; may also be used at reduced concentration in high-fidelity PCR. |
| Application Notes / Precautions | The enhancer may reduce PCR fidelity. Use 0.5–1× final concentration when high-fidelity amplification is required. |
For research use only, not for clinical use.
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