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488-EdU Cell Proliferation Assay Kit

Cat.No: CAAK-0009 Datasheet

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Product Name 488-EdU Cell Proliferation Assay Kit
Catalog No. CAAK-0009
Description A green-fluorescence EdU assay for measuring DNA synthesis and cell proliferation at single-cell resolution using a 488-channel fluorescent azide probe.
Intended Use Cell proliferation, cell-viability and cytotoxicity studies.
Principle / Technology EdU is incorporated into replicating DNA and detected through a copper-catalyzed covalent click reaction with a green fluorescent azide probe.
Detection Method Fluorescence microscopy and flow cytometry.
Sample Type Proliferating cultured cells.
Performance Range / Specifications 100-reaction format with 488-channel fluorescent detection.
Specificity Strong fluorescence with low staining background supports accurate detection of proliferating cells.
Reaction Conditions / Protocol Pulse cells with EdU, fix and permeabilize, perform the fluorescent click reaction, wash and analyze by imaging or flow cytometry.
Components / Formulation EdU labeling reagent and 488-channel fluorescent azide click-reaction components.
Storage Conditions Store at -30 to -15°C protected from light.
Package Specifications 100 reactions.
Product Form Green fluorescent EdU cell-proliferation kit.
Key Features Single-cell proliferation tracing; strong green fluorescence; low background; compatible with microscopy and flow cytometry.
Shipping Conditions Ship at 0°C or below.
Compatibility Fluorescence imaging systems and flow cytometers with a suitable 488-channel configuration.
Application Notes / Precautions Protect fluorescent reagents from light, wash thoroughly after staining and avoid overexposure during image acquisition.

For research use only, not for clinical use.

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