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| Product Name | 300 nm Anti-DYKDDDDK Magnetic Beads at 10 mg/mL for Tagged Proteins |
| Catalog No. | IRMGM-0049 |
| Description | Alta DiagnoTech anti-DYKDDDDK magnetic beads use a rabbit monoclonal antibody covalently coupled to nanoscale carboxyl magnetic beads. The superparamagnetic particles selectively capture DYKDDDDK-tagged fusion proteins and associated complexes from lysates, serum or ascites. |
| Intended Use | Immunoprecipitation, co-immunoprecipitation and purification of DYKDDDDK- or FLAG-tagged proteins and complexes. |
| Principle / Technology | Monoclonal-antibody affinity capture followed by magnetic separation. |
| Sample Type | Animal, plant or microbial lysate, serum and ascites |
| Specificity | Met-DYKDDDDK-protein, DYKDDDDK-protein and protein-DYKDDDDK configurations |
| Reaction Conditions / Protocol | Performance example: 20 µL beads with 500 µL sample containing about 0.7 mg total protein; incubate overnight at 4 °C. Elute with 20 µL 1× SDS-PAGE loading buffer by heating at 100 °C for 5 minutes, then collect the supernatant for Western blot analysis. |
| Components / Formulation | Rabbit anti-DYKDDDDK-tag monoclonal antibody covalently coupled to carboxyl magnetic beads; antibody isotype IgG1 |
| Package Specifications | 0.5 mL, 1 mL or 5 mL |
| Product Form | Anti-DYKDDDDK antibody magnetic beads |
| Key Features | Approximately 300 nm particles High loading with 10-20 µL beads per experiment Low nonspecific background Three elution approaches |
| Concentration | 10 mg/mL in protective buffer |
| Usage Method | Elute with SDS-PAGE loading buffer, acidic elution or competitive peptide elution. |
| Product Type | Epitope-tag affinity magnetic beads |
| Function / Efficacy | Affinity capture and purification of DYKDDDDK-tagged proteins and complexes. |
| Analytes / Targets | DYKDDDDK- or FLAG-tagged fusion proteins and protein complexes |
| Matrix | Nanoscale carboxyl magnetic beads |
| Particle Size | Approximately 300 nm |
| Ligand / Bonded Phase | Rabbit anti-DYKDDDDK-tag monoclonal antibody |
| Ligand Coupling Method | Covalent coupling to carboxyl magnetic beads |
For research use only, not for clinical use.
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