300 nm-150 µm NHS-Activated Polymer and Agarose Magnetic Beads

Name: 300 nm-150 µm NHS-Activated Polymer and Agarose Magnetic Beads
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Cat.No: IRMGM-0062 Datasheet

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Product Name	300 nm-150 µm NHS-Activated Polymer and Agarose Magnetic Beads
Catalog No.	IRMGM-0062
Description	Alta DiagnoTech NHS-activated magnetic media couple proteins, antibodies, enzymes, peptides and nucleic acids through stable amide bonds under mild conditions.
Intended Use	Covalent immobilization of primary-amine biomolecules for assays, capture, immunoprecipitation and purification.
Principle / Technology	Surface NHS groups react directly with primary amines to form stable amide bonds without separate EDC/NHS or glutaraldehyde activation.
Sample Type	Primary-amine-containing proteins, antibodies, antigens, enzymes, peptides and nucleic acids
Performance Range / Specifications	Ligand-coupling efficiency can reach at least 90%. Coupling is completed in 1-2 hours at room temperature or 4 °C over pH 5-9.
Reaction Conditions / Protocol	Dissolve the amine-containing ligand in coupling buffer and mix with the NHS beads for 1-2 hours at room temperature; 4 °C coupling is also supported. Recommended coupling pH is 5-9.
Package Specifications	2 µm polymer beads at 10 mg/mL: 1 or 5 mL kits and 50 mL bulk format; 10-30 µm agarose beads at 20% v/v: 1 or 5 mL kits and 50 or 1,000 mL bulk formats; 30-150 µm agarose beads at 20% v/v: 1, 5, 50 or 1,000 mL; 300 nm polymer beads at 10 mg/mL: 1 or 10 mL.
Product Form	NHS-activated magnetic bead suspension or coupling kit
Key Features	No separate activation step, rapid high-efficiency coupling, mild reaction conditions, stable amide linkage and low nonspecific adsorption.
Concentration	Polymer formats: 10 mg/mL; agarose formats: 20% v/v
Compatibility	IVD development, immunoassays, cell sorting, IP/Co-IP and protein or antibody purification
Product Type	NHS-activated magnetic coupling media
Function / Efficacy	Covalently immobilize primary-amine biomolecules on magnetic particles.
Functional Group	N-hydroxysuccinimide ester
Matrix	Solid polymer microspheres or porous agarose microspheres
Particle Size	300 nm, 2 µm, 10-30 µm or 30-150 µm
Ligand / Bonded Phase	NHS-activated surface
Ligand Coupling Method	Direct primary-amine coupling to form a stable amide bond
pH Stability	Coupling range pH 5-9

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For research use only, not for clinical use.