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| Product Name | 30-150 µm Glutathione Magnetic Beads for GST-Fusion Protein Purification |
| Catalog No. | IRMGM-0061 |
| Description | Alta DiagnoTech glutathione-functional magnetic agarose enables rapid one-step capture and elution of GST-fusion proteins without column packing, pressure or flow-rate control. |
| Intended Use | Research and process-scale magnetic purification of GST-fusion proteins. |
| Principle / Technology | Glutathione affinity capture of GST-tagged proteins on magnetic agarose beads. |
| Detection Method | Magnetic affinity purification |
| Sample Type | Crude biological protein samples containing GST-fusion proteins |
| Performance Range / Specifications | Target binding capacity reaches 10 mg/mL of fully settled beads. Parallel-operation CV values are 1.6% for purity and 4.5% for loading; between-batch CV values are 0.7% for purity and 4.7% for loading. Binding performance and purity remained stable across five reuse cycles in the displayed evaluation. |
| Reaction Conditions / Protocol | The displayed elution comparison uses 0.2, 0.5, 1 and 10 mM glutathione buffers. A complete purification can be completed within approximately 1 hour. |
| Storage Conditions | 4-30 °C; 4-8 °C is recommended for long-term storage |
| Package Specifications | 5 mL, 2 × 50 mL or 4 × 250 mL bead formats at 10% v/v and 30-150 µm; a 5 mL purification-kit format is also listed |
| Product Form | Magnetic agarose bead suspension or purification kit |
| Key Features | One-step purification, no column hardware, flexible sample scale, parallel high-throughput processing, adjustable elution volume and reusable media. |
| Concentration | 10% v/v bead suspension |
| Batch-to-Batch Consistency | CV 0.7% for target-protein purity and 4.7% for loading in the displayed lot comparison |
| Product Type | GST-affinity magnetic agarose beads |
| Function / Efficacy | Capture and purify GST-fusion proteins by magnetic separation. |
| Storage Buffer | 20% v/v ethanol |
| Analytes / Targets | GST-fusion proteins |
| Matrix | Magnetic agarose |
| Particle Size | 30-150 µm |
| Ligand / Bonded Phase | Glutathione |
| Dynamic Binding Capacity | Up to 10 mg GST-fusion protein per mL of fully settled beads |
| Chemical Stability | At room temperature, tolerates 70% ethanol, 6 M guanidine hydrochloride, 0.1 M sodium hydroxide or 0.1 M acetic acid for 1 hour. |
| Regeneration Conditions | Cleaning is recommended after approximately three to five reuse cycles. |
For research use only, not for clinical use.
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