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| Product Name | 24,25-DVD3 (24,25-Dihydroxy Vitamin D3) ELISA Kit |
| Catalog No. | HTK-YJL-0004 |
| Synonyms | 24,25-DVD3 ELISA Kit, 24,25-Dihydroxy Vitamin D3 ELISA Kit |
| Species | Universal |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.781-50 ng/ml |
| Sensitivity | 0.469 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with 24,25-DVD3, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
24,25-Dihydroxy Vitamin D3 (24,25-DVD3) is a critical metabolite of Vitamin D3, playing a key role in the intricate regulation of calcium and phosphorus homeostasis within the human and animal body. Unlike the more widely recognized 25-Hydroxy Vitamin D3, 24,25-DVD3 is primarily involved in the feedback loop that modulates Vitamin D activity—acting as a "regulator" to prevent excessive activation of Vitamin D-responsive pathways, which is essential for maintaining normal bone health, immune function, and cellular metabolism.
In research settings, the accurate detection of 24,25-DVD3 levels has become increasingly important for several fields:
Bone Metabolism Studies: Abnormal 24,25-DVD3 concentrations are associated with conditions like osteoporosis, osteomalacia, and renal osteodystrophy, as the metabolite reflects the body’s ability to balance Vitamin D utilization for bone mineralization.
Renal Function Research: The kidneys are the primary site for 24,25-DVD3 synthesis. Impaired renal function (e.g., chronic kidney disease) often disrupts 24,25-DVD3 production, making it a valuable biomarker for assessing renal metabolic health.
Vitamin D Toxicity and Deficiency Research: Measuring 24,25-DVD3 alongside other Vitamin D metabolites (e.g., 25(OH)D3, 1,25(OH)2D3) helps distinguish between true Vitamin D deficiency and cases where metabolic dysregulation (rather than insufficient intake) drives low active Vitamin D levels. It also aids in monitoring patients at risk of Vitamin D toxicity, as elevated 24,25-DVD3 may indicate excessive Vitamin D accumulation.
Traditional methods for 24,25-DVD3 detection, such as high-performance liquid chromatography (HPLC) or liquid chromatography-tandem mass spectrometry (LC-MS/MS), offer high accuracy but require expensive equipment, specialized technical expertise, and long processing times—limiting their accessibility for routine research or high-throughput sample analysis.
The 24,25-DVD3 ELISA Kit addresses these challenges by leveraging the specificity of antigen-antibody interactions and the efficiency of enzyme-linked immunosorbent assay (ELISA) technology. It provides a cost-effective, user-friendly, and time-efficient alternative, enabling researchers across academic labs, pharmaceutical companies, and clinical research institutions to quantify 24,25-DVD3 levels in diverse biological samples with minimal equipment requirements.
Universal Species Compatibility: Designed to detect 24,25-DVD3 across a wide range of species (including human, mouse, rat, rabbit, and other common research models), eliminating the need to purchase species-specific kits and simplifying multi-species study designs.
Versatile Sample Compatibility: Validated for use with multiple biological sample types—serum, plasma, cell culture supernatant, and cell/tissue lysate—allowing researchers to analyze 24,25-DVD3 in different experimental contexts without sample pre-treatment limitations.
Rapid Assay Workflow: Completes the entire detection process (from sample addition to result reading) in just 2 hours, significantly reducing assay time compared to HPLC/LC-MS/MS methods (which typically take 4–8 hours) and enabling high-throughput sample processing.
Antigen-Coated Competitive ELISA Design: Utilizes a pre-coated antigen format for the competitive ELISA, ensuring consistent capture antibody density across all wells, minimizing inter-assay variability, and simplifying experimental setup (no need for manual antibody coating).
Precise Detection Range: Offers a detection range of 0.781–50 ng/ml, which aligns with the physiological concentration range of 24,25-DVD3 in most biological samples (e.g., 1–15 ng/ml in human serum), ensuring accurate quantification of both normal and abnormal levels.
High Sensitivity: Achieves a sensitivity of 0.469 ng/ml, enabling the detection of low 24,25-DVD3 concentrations in samples from research models with impaired Vitamin D metabolism (e.g., renal disease models) where metabolite levels may be below the detection limit of less sensitive kits.
Stable Storage Conditions: Requires only 2–8°C storage (no cryopreservation needed) for long-term stability, reducing the risk of reagent degradation during storage and simplifying inventory management.
Exceptional Specificity: Specifically binds to 24,25-DVD3 with no obvious cross-reactivity with other Vitamin D analogues (e.g., 25-Hydroxy Vitamin D3, 1,25-Dihydroxy Vitamin D3) or common biological interferents (e.g., lipids, proteins in serum), ensuring reliable and accurate results that are not skewed by sample matrix components.
User-Friendly Operation: Does not require specialized technical skills or advanced equipment—only a standard microplate reader (capable of reading OD450 nm) is needed. The kit includes all essential components (pre-coated plates, standards, buffers, substrates) and a detailed manual, enabling even novice users to perform assays with minimal training.
Cost-Effective: Offers a lower per-sample cost compared to HPLC/LC-MS/MS methods, as it eliminates the need for expensive instrumentation, consumables (e.g., HPLC columns), or specialized maintenance. It also reduces labor costs by streamlining the assay workflow.
Reproducible Results: The pre-coated plate design and optimized reagent formulations minimize intra-assay (within a single plate) and inter-assay (across multiple plates) variability, ensuring consistent results that can be replicated across experiments and labs—critical for validating research findings.
Flexible Format Options: Available in 48T and 96T formats, allowing researchers to choose the size that matches their sample volume needs (e.g., 48T for small-scale pilot studies, 96T for high-throughput experiments), reducing reagent waste and optimizing cost efficiency.
For research use only, not for clinical use.
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