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| Product Name | Chicken CCK (Cholecystokinin) ELISA Kit |
| Catalog No. | HTK-YJL-0038 |
| Synonyms | CCK ELISA Kit, Cholecystokinin ELISA Kit |
| Species | Chicken |
| UniProt ID | Q9PU41 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 15.625-1000 pg/ml |
| Sensitivity | 9.375 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with CCK, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Cholecystokinin (CCK) is a key gastrointestinal peptide hormone with multifunctional roles in both the digestive system and central nervous system, and its detection in chicken models is critical for advancing poultry science, veterinary research, and agricultural biotechnology.
In poultry physiology research, chicken CCK plays a pivotal role in regulating feed intake, nutrient digestion, and gut health—core factors that directly impact poultry growth performance, flock productivity, and farm economic efficiency. For instance, fluctuations in chicken CCK levels are closely linked to changes in appetite: elevated CCK concentrations often signal satiety, while reduced levels may indicate increased feed consumption, making it a vital biomarker for studying poultry feeding behavior and optimizing dietary formulations.
Beyond digestive regulation, chicken CCK is involved in modulating gut-brain communication, pancreatic enzyme secretion (such as amylase and lipase), and gallbladder contraction in chickens—processes that are essential for maintaining normal digestive function and nutrient absorption. In pathological research, abnormal CCK expression in chickens has been associated with gut disorders (e.g., enteritis), metabolic imbalances, and even stress-induced physiological changes, making it a valuable indicator for diagnosing and studying poultry digestive diseases.
With the growing demand for sustainable poultry farming, improved animal welfare, and high-quality poultry products, research on chicken CCK has become increasingly prominent. Traditional methods for CCK detection (such as radioimmunoassay) often suffer from limitations including high radiation risk, long detection cycles, and poor specificity for chicken-derived CCK. The Chicken CCK ELISA Kit addresses these gaps by providing a safe, rapid, and species-specific solution, enabling researchers, veterinary laboratories, and agricultural biotech institutions to accurately quantify CCK levels in various chicken samples and drive advancements in poultry research.
Species-Specific Detection: Precisely targets chicken CCK (UniProt ID: Q9PU41) with no obvious cross-reactivity with CCK analogues from other species (e.g., mouse, rat, or human) or other chicken peptides, ensuring the accuracy of experimental results in chicken-specific research.
Broad Sample Compatibility: Designed to work with multiple common chicken sample types, including serum, plasma, cell culture supernatant, and cell/tissue lysate. This flexibility eliminates the need for researchers to purchase separate kits for different sample matrices, simplifying experimental workflows.
Optimal Detection Range: Covers a linear range of 15.625–1000 pg/ml, which aligns with the typical physiological concentration of CCK in chicken samples (as reported in poultry physiology studies). This range ensures that both low and high CCK levels can be quantified without the need for excessive sample dilution or concentration.
High Sensitivity: Boasts a detection limit of 9.375 pg/ml, allowing for the accurate measurement of low-abundance CCK in chicken samples (e.g., early-stage gut disorder models or subtle changes in CCK due to dietary adjustments), which is critical for capturing minor physiological or pathological fluctuations.
Rapid Turnaround Time: Completes the entire detection process in just 4 hours, significantly faster than traditional CCK detection methods (which often take 8–12 hours). This reduces experimental waiting time and enables researchers to accelerate data collection and project progress.
Stable Storage Conditions: Requires only 2–8°C refrigerated storage (no cryopreservation needed), minimizing the risk of reagent degradation during storage and transportation. This stability also simplifies inventory management for laboratories without specialized ultra-low temperature equipment.
Reliable Result Reproducibility: Utilizes the double-antibody sandwich ELISA technique, a gold standard in immunoassay technology, which ensures high intra-assay and inter-assay precision. Studies show that this method delivers consistent results with a coefficient of variation (CV) of less than 10%, reducing experimental errors and enhancing data credibility for research publications.
User-Friendly Operation: Comes with pre-optimized reagents and a detailed, step-by-step protocol, requiring no advanced technical skills to operate. Even researchers with limited ELISA experience can complete the assay successfully, lowering the barrier to use for small laboratories or new research teams.
Cost-Effective Format Options: Available in 48T and 96T formats, allowing laboratories to choose the appropriate size based on their sample volume (e.g., 48T for small-scale pilot studies, 96T for large-scale experiments). This flexibility helps reduce unnecessary reagent waste and lowers research costs.
Safe to Use: Eliminates the radiation risk associated with radioimmunoassay kits, complying with standard laboratory safety regulations. The kit’s reagents are non-toxic and easy to handle, ensuring the safety of laboratory personnel during operation.
Validated for Research Applications: Specifically developed for research use in poultry physiology, veterinary medicine, and agricultural biotechnology—fields where chicken CCK studies are in high demand. The kit’s performance has been validated with multiple chicken sample types, ensuring its suitability for a wide range of research projects (e.g., dietary impact studies, gut health research, stress response analysis).
For research use only, not for clinical use.
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